青钱柳等几种别样茶中咖啡因、可可碱和茶碱的检测
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引用本文:何春年,彭勇,肖伟,肖培根.青钱柳等几种别样茶中咖啡因、可可碱和茶碱的检测[J].中国现代中药,2012,14(4):14-17
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作者中文名作者英文名单位中文名单位英文名E-Mail
何春年 HE Chun-nian 中国医学科学院 北京协和医学院 药用植物研究所,北京 100193 Institute of Medicinal Plant Development,Chinese Academy of Medical Science,Peking Union Medical College,Beijing 100193,China  
彭勇 PENG Yong 国家教育部中草药物质基础与资源利用重点实验室,北京 100193 Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine Peking Union Medical College,Ministry of Education,Beijing 100193,China  
肖伟 XIAO Wei 中国医学科学院 北京协和医学院 药用植物研究所,北京 100193 Institute of Medicinal Plant Development,Chinese Academy of Medical Science,Peking Union Medical College,Beijing 100193,China  
肖培根 XIAO Pei-gen 国家教育部中草药物质基础与资源利用重点实验室,北京 100193 Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine Peking Union Medical College,Ministry of Education,Beijing 100193,China xiaopg@public.bta.net.cn 
中文摘要:对青钱柳甜茶等几种别样茶中咖啡因、可可碱和茶碱进行检测,为别样茶的合理应用奠定基础。方法:采用超快速液相色谱法(RSLC),Acclaim PA2色谱柱(150 mm×2.1 mm,2.2 μm),乙腈-水梯度洗脱;流速:0.5 mL·min-1;检测波长:273 nm,柱温:30 ℃。结果:除加入绿茶的商品中检测到适量的咖啡因外,青钱柳神茶、黄芩茶、多穗柯甜茶、悬钩子属甜茶,以及甜叶菊叶和湖北海棠叶中均未检测出上述3种生物碱。结论:相对于绿茶中含有较高含量的咖啡因、可可碱和茶碱,别样茶中未检出上述3种成分,为别样茶的科学应用提供指导。
中文关键词:别样茶  青钱柳  咖啡因  可可碱  茶碱  RSLC
 
Determination of Caffeine,Theobromine,and Theophylline in the Leaves of Cyclocarya paliurus and Several Other Kinds of Tea
Abstract:To determine caffeine,theobromine,and theophylline contents in the leaves of Cyclocarya paliurus and several other kinds of tea by RSLC.Methods:The separation was carried out through a gradient elution using a Dionex Acclaim RSLC PA2(150 mm×2.1 mm,2.2 μm) column and a mobile phase consisting of water and acetonitrile at a flow rate of 0.5 mL·min-1.The detection wavelength was set at 273 nm.The column temperature was 30 ℃.Results:Under the optimized conditions,all the 3 analytes were successfully separated within 10 min,and good linearity(r>0.999 6)was achieved.This method was also validated with respect to precision,specificity,and was successfully applied to simultaneous determination of 3 compounds in the leaves of Cyclocarya paliurus and several other kinds of tea including:Huang-qin tea,Duo-sui-ke tea,Xuan-gou-zi sweet tea,Tian-ye-ju tea,Hubei-hai-tang tea.The results indicate that the 3 compounds were not detected in the six species of plants.Conclusion:It can guide the application of science for other kinds of tea.
keywords:Cyclocarya paliurus  Caffeine  Theobromine  Theophylline  RSLC
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