Identification of Plants in Macleaya spp.Based on ITS2 Sequence
Abstract:Objective:To identify the medicinal plant Macleaya spp.by using ITS2 sequence.Methods:The ITS2 sequence and sequence of 30 samples collected from Hunan,Jiangxi,Zhejiang,Hubei,Henan,Shanxi,Chongqing was amplified and the amplification efficiency,the sequencing success rate,the constitution of base pair,intra- and inter-specific variation and NJ(Neighbor-Joining) tree were analyzed,in order to evaluate the ITS2 sequencing results.Results:The ITS2 sequence length of Macleaya spp.was within 500 bp;the amplification efficiency and the success sequencing rate was 100% and 30 ITS2 sequences were obtained.Conclusion:The ITS2 sequence can be effective and accurate for identification of Macleaya spp..