在模拟人工胃肠道环境中博落回主要生物碱类成分的稳定性特征分析
投稿时间:2017-02-22     点此下载全文
引用本文:张换成,陈梅,柳亦松,唐昭山,曾建国,刘兆颖.在模拟人工胃肠道环境中博落回主要生物碱类成分的稳定性特征分析[J].中国现代中药,2017,19(10):1387-1390
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作者中文名作者英文名单位中文名单位英文名E-Mail
张换成 ZHANG Huancheng 湖南农业大学 兽用中药资源与中兽药创制国家地方联合工程研究中心,湖南长沙410128 National and Local Union Engineering Research Center for the Veterinary Herbal Medicine Resources and Initiative,Hunan Agricultural University,Changsha 410128,China  
陈梅 CHEN Mei 湖南农业大学 兽用中药资源与中兽药创制国家地方联合工程研究中心,湖南长沙410128 National and Local Union Engineering Research Center for the Veterinary Herbal Medicine Resources and Initiative,Hunan Agricultural University,Changsha 410128,China  
柳亦松 LIU Yisong 湖南农业大学 兽用中药资源与中兽药创制国家地方联合工程研究中心,湖南长沙410128 National and Local Union Engineering Research Center for the Veterinary Herbal Medicine Resources and Initiative,Hunan Agricultural University,Changsha 410128,China  
唐昭山 TANG Zhaoshan 湖南美可达生物资源股份有限公司,湖南长沙410005 Micolta Biological Resource INC,Changsha 410005,China  
曾建国 ZENG Jianguo 湖南农业大学 兽用中药资源与中兽药创制国家地方联合工程研究中心,湖南长沙410128 National and Local Union Engineering Research Center for the Veterinary Herbal Medicine Resources and Initiative,Hunan Agricultural University,Changsha 410128,China 曾建国,博士生导师,教授,研究方向:中药资源与综合利用,Tel:(0731)84673824,E-mail:zengjianguo@hunau.edu.cn 
刘兆颖 LIU Zhaoying 湖南农业大学 兽用中药资源与中兽药创制国家地方联合工程研究中心,湖南长沙410128 National and Local Union Engineering Research Center for the Veterinary Herbal Medicine Resources and Initiative,Hunan Agricultural University,Changsha 410128,China 刘兆颖,硕士生导师,副教授,研究方向:兽医药理学与毒理学,E-mail:liu_zhaoying@sina.com 
基金项目:湖南省科技重点计划(2016SK3002);湖南省自然科学基金(2017JJ1017)
中文摘要:目的:为解释博落回中4个生物碱(原阿片碱、别隐品碱、血根碱、白屈菜红碱)生物利用低的原因,对这四个生物碱在模拟人工胃肠道环境下的稳定性进行考察。方法:采用HPLC测定博落回中4个生物碱在不同pH缓冲液、人工胃液(pH=1.3)和人工肠液(pH=6.8)中的稳定性。结果:原阿片碱和别隐品碱在酸性、中性、碱性、人工胃液及人工肠液环境下8 h回收率为97.16%~101.89%;血根碱在pH=6.8磷酸盐缓冲液、水、pH=7.8~8.0 磷酸盐缓冲液及人工肠液中8 h回收率为48.81%~60.53%;白屈菜红碱在pH=5.8磷酸盐缓冲液、pH=6.8磷酸盐缓冲液、水及人工肠液中8 h的回收率为81.21%~93.48%;在pH=7.8~8.0 磷酸盐缓冲液中8 h回收率为57.43%。结论:原阿片碱和别隐品碱的稳定性不受pH的影响,血根碱和白屈菜红碱随pH升高稳定性降低,博落回中4个生物碱在模拟人工胃肠道环境中的稳定性结果与其在不同pH环境中一致,为阐明博落回散在体内的药代动力学特点提供科学依据。
中文关键词:博落回  血根碱  白屈菜红碱  胃肠道稳定性
 
Stability Analysis of Major Alkaloids in Macleaya cordata under Simulated Gastrointestinal Environment
Abstract:Objective:In order to explain the reasons of low bioavailability of four alkaloids (protopine,allocryptopine,sanguinarine,chelerythrine) in Macleaya cordata,the study explored the stability of four alkaloids in the simulated gastrointestinal environment.Methods:A high performance liquid chromatography method was used to determine the stability of four alkaloids in M.cordata under the environment of different pH media,artificial gastric juice (pH=1.3) and artificial intestinal juice (pH=6.8).Results:The recovery rate of protopine and allocryptopine were ranging from 97.16% to 101.89% in the environment of acidic,neutral,alkalinity media,artificial gastric juice and artificial intestinal juice for 8 h.The recovery rate of sanguinarine was 48.81%-60.53% in the environment of pH=6.8 phosphate buffer solution (PBS),water,pH=7.8-8.0 PBS and artificial intestinal juice for 8 h.The recovery rate of chelerythrine was 81.21%-93.48% in the environment of pH=5.8 PBS,pH=6.8 PBS,water and artificial intestinal juice for 8 h,while in the environment of pH=7.8-8.0 PBS for 8 h,the recovery rate of chelerythrine was 57.43%.Conclusion:The stability of protopine and allocryptopine was not affected by pH.While along with the increase of pH,the stability of sanguinarine and chelerythrine was reduced.The stability of four alkaloids in M.cordata under the simulated gastrointestinal environment accorded with the environment of different pH media.The results provide a scientific basis for clarifying the pharmacokinetic characteristics of Sangrovit.
keywords:Macleaya cordata  sanguinarine  chelerythrine  gastrointestinal tract stability
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