血根碱对大鼠结肠炎及炎症通路中相关因子的影响 |
投稿时间:2017-02-26 点此下载全文
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引用本文:柳亦松,唐昭山,刘兆颖,蒋昊航,段志贵,曾建国.血根碱对大鼠结肠炎及炎症通路中相关因子的影响[J].中国现代中药,2017,19(10):1391-1396 |
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基金项目:国家自然科学基金(31200615);国家重点实验室培育基地开放项目(15KFXM09) |
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中文摘要:目的:探讨血根碱对葡聚糖硫酸钠致溃疡性结肠炎的疗效及可能的机制。方法:建立葡聚糖硫酸钠致溃疡性结肠炎大鼠模型,随机分为空白组、模型组、柳氮磺吡啶组、血根碱组。观察各组大鼠体质量、腹泻及隐血情况;通过荧光定量PCR(qPCR)分析炎症因子IL-1β、IL-6、IL-10、TNF-α在结肠组织中的表达量;利用免疫组化实验检测NF-κB通路中关键因子p65。结果:与模型组相比,血根碱组和阳性组大鼠的结肠组织中炎症因子IL-1β、IL-6表达降低,而IL-10的表达升高;同时,免疫组化实验发现血根碱组与模型组相比,其核内转入因子核蛋白p65显著下调。结论:血根碱可能通过调节NF-κB通路中的核转入因子p65,降低炎症因子IL-1β、IL-6的表达,同时提高炎症因子IL-10的表达,从而对炎症起到抑制作用。 |
中文关键词:血根碱 结肠炎 炎症因子 NF-κΒ(p65) |
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Effects of Sanguinarine on Inflammatory Factors in Rat Colitis and Inflammatory Pathway |
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Abstract:Objective:To explore the regulatory effect of sanguinarine on inflammation,and provide the foundation for research of sanguinarine for its anti-inflammatory effect.Methods:With dextran sulfate sodium induced colitis in rats as a model,the regulating mechanism of sanguinarine on rat body weight,diarrhea and colitis and the occult blood detection factor was observed.The expression of IL-1,IL-6,IL-10 and TNF-α in colonic tissue was analyzed by fluorescence quantitative PCR (qPCR).Results:Compared with the model group,the nuclear factor p65 protein in the sanguinarine group significantly decreased.Conclusion:Sanguinarine can up-regulate IL-10 and down-regulate IL-1β,IL-6,and inhibits inflammation.The nuclear factor p65 was also down-regulated. |
keywords:Sanguinarine colitis inflammatory factors NF-κΒ(p65) |
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