不同产地博落回离体培养与植株再生研究 |
投稿时间:2017-02-20 点此下载全文
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引用本文:徐敏,宋锡帅,黄鹏,唐其,唐昭山,刘薇.不同产地博落回离体培养与植株再生研究[J].中国现代中药,2017,19(10):1420-1423 |
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基金项目:湖南省科技重点计划(2016SK3002);湖南省教育厅科学研究项目(16C0769);博落回血根碱/白屈菜红碱生物合成关键酶-P6H的功能研究(2016JJ4040) |
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中文摘要:目的:探索不同产地的博落回离体培养效果差异,为筛选最适博落回植株再生材料提供研究基础。方法:采用植物组织培养技术对博落回叶片组织进行愈伤诱导及胚状体分化,并对结果进行分析。结果:安徽祁门、湖南炎陵和湖南高坊产地分别在不同培养阶段与其他产地比诱导率和分化率最高。结论:湖南高坊和安徽祁门的愈伤组织和胚状体的诱导及分化能力优于其他产地的样本。因此,不同产地叶片离体培养效果差异显著。 |
中文关键词:博落回,体细胞胚胎发生,离体培养,植株再生 |
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Study on Vitro Culture and Plant Regeneration of Macleaya cordata |
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Abstract:Objective:To explore the differenle of different regions vitro culture of Macleaya cordata and provide data for screening the optimal plant regeneration materials.Methods:The leaves of M.cordata were isolated to callus induction and differentiation of leaves into embryos by using plant cell culture technology and the results differentiation was analyzed.Results:The samples from Qimen of Anhui,Yanling of Hunan and Gaofang of Hunan had the highest induction and differentiation rate compared with others in different periods.Conclusion:The induction and the differentiation ability of callus and embryos bodies from Gaofang of Hunan and Qimen of Anhui were superior to those from other regions.Therefore,the vitro culture effect of leaves from different regions was significant difference. |
keywords:Macleaya cordata,somatic embryo genesis,vitro culture,plant regeneration |
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