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作者中文名	作者英文名	单位中文名	单位英文名	E-Mail
黄维	HUANG Wei	福建农林大学生命科学学院，福建福州350002	College of Life Sciences，Fujian Agriculture and Forestry University，Fuzhou 350002，China	黄维，讲师，研究方向：中药学；E-mail：huangwei@fafu.edu.cn
邹小兴	ZOU Xiao-xing	自然生物资源保育利用福建省高校工程研究中心，福建福州350002	Engineering Research Institute of Conservation，Utilization of Natural Bioresources，Fujian Agriculture and Forestry University，Fuzhou 350002，China
丁卉	DING Hui	自然生物资源保育利用福建省高校工程研究中心，福建福州350002	Engineering Research Institute of Conservation，Utilization of Natural Bioresources，Fujian Agriculture and Forestry University，Fuzhou 350002，China
梁文贤	LIANG Wen-xian	自然生物资源保育利用福建省高校工程研究中心，福建福州350002	Engineering Research Institute of Conservation，Utilization of Natural Bioresources，Fujian Agriculture and Forestry University，Fuzhou 350002，China
倪林	NI Lin	自然生物资源保育利用福建省高校工程研究中心，福建福州350002	Engineering Research Institute of Conservation，Utilization of Natural Bioresources，Fujian Agriculture and Forestry University，Fuzhou 350002，China
邹双全	ZOU Shuang-quan	自然生物资源保育利用福建省高校工程研究中心，福建福州350002	Engineering Research Institute of Conservation，Utilization of Natural Bioresources，Fujian Agriculture and Forestry University，Fuzhou 350002，China	邹双全，研究员，研究方向：药用观赏植物；E-mail：zou@fafu.edu.cn

基金项目:国家农业科技成果转化资金项目（2010GB2C00216）；福建省自然科学基金（13181082）；福建农林大学科技创新专项基金项目（CXZX2016072，CXZX2016073，CXZX2016074）

中文摘要:目的：探讨圆齿野鸦椿醇提物的抗肝癌活性。方法：制备圆齿野鸦椿果皮、枝条、叶片和种子的乙醇提取物，采用MTT法体外检测这4种醇提物对人肝癌细胞HepG2、SMMC-7721和BEL-7404的增殖抑制作用，流式细胞法检测上述4种醇提物体外诱导HepG2细胞凋亡的情况，采用小鼠肝癌H22移植瘤模型进行体内抑瘤实验，探讨圆齿野鸦椿各部位醇提物的抗肝癌效果。结果：圆齿野鸦椿果皮、枝条、叶片和种子的乙醇提取物处理细胞48 h后，发现果皮醇提物对HepG2、SMMC-7721和BEL-7404肝癌细胞的抑制效果最好，其半数抑制浓度IC50可分别达到108.34、106.94、114.79 μg·mL-1。采用质量浓度为200 μg·mL-1的圆齿野鸦椿果皮、枝条、叶片和种子醇提物，处理HepG2细胞48 h，均可诱导HepG2细胞凋亡，其中以果皮醇提物的效果最好。体内实验以圆齿野鸦椿果皮、枝条、叶片和种子醇提物200 mg·kg-1对H22移植瘤小鼠灌胃给药14 d，其抑瘤率可分别达到47.8%、44.9%、42.5%和40.6%。结论：圆齿野鸦椿的乙醇提取物具有良好的抗肝癌作用。

中文关键词:圆齿野鸦椿乙醇提取物抗肝癌

Research on Anti-hepatoma Effect of Euscaphis konishii Hayata Ethanol Extracts

Abstract:Objective：To study the anti-hepatoma effect of the ethanol extracts of Euscaphis konishii Hayata.Methods：The E.konishii pericarps，leaves，branches and seeds were extracted by ethanol.The anti-proliferation activities in vitro were detected by MTT assay against human liver cancer cells HepG2，SMMC-7721 and BEL-7404.Flow cytometry was used to detect the HepG2 cell apoptosis. Mice bearing H22 murine hepatoma xenograft model was established to evaluate the anti-tumor activity in vivo.Results：After 48 h treatment of HepG2，SMMC-7721 and BEL-7404 cells with the ethanol extracts of E.konishii pericarps，leaves，branches and seeds，MTT assay showed that the pericarps extract demonstrated the best anti-hepatoma effect in vitro，with the half inhibitory concentration（IC50）of 108.34，106.94 and 114.79 μg·mL-1.The results of flow cytometry showed that all the four extracts could obviously induce HepG2 cell apoptosis，and the pericarps extract had the best effect.H22 murine hepatoma xenograft test was carried out with 200 mg·kg-1 of the pericarps，leaves，branches and seeds extract for 14 d by gavage administration，with the anti-tumor rate of 47.8%，44.9%，42.5% and 40.6%，respectively.Conclusion：The ethanol extract of E.konishii has obvious anti-hepatoma activity.

keywords:Euscaphis konishii Hayata,ethanol extract,anti-hepatoma

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