三株甲基营养型芽孢杆菌抑菌活性物质初探
投稿时间:2017-09-14     点此下载全文
引用本文:邵天蔚,张晓云,丁万隆,王蓉,李勇.三株甲基营养型芽孢杆菌抑菌活性物质初探[J].中国现代中药,2018,20(2):189-194
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作者中文名作者英文名单位中文名单位英文名E-Mail
邵天蔚 SHAO Tian-wei 中国医学科学院 北京协和医学院 药用植物研究所,北京100193 Institute of Medicinal Plant Development,Beijing Union Medical College & Chinese Academy of Medical Sciences,Beijing 100193,China  
张晓云 ZHANG Xiao-yun 中国医学科学院 北京协和医学院 药用植物研究所,北京100193 Institute of Medicinal Plant Development,Beijing Union Medical College & Chinese Academy of Medical Sciences,Beijing 100193,China  
丁万隆 DING Wan-long 中国医学科学院 北京协和医学院 药用植物研究所,北京100193 Institute of Medicinal Plant Development,Beijing Union Medical College & Chinese Academy of Medical Sciences,Beijing 100193,China  
王蓉 WANG Rong 中国医学科学院 北京协和医学院 药用植物研究所,北京100193 Institute of Medicinal Plant Development,Beijing Union Medical College & Chinese Academy of Medical Sciences,Beijing 100193,China  
李勇 LI Yong 中国医学科学院 北京协和医学院 药用植物研究所,北京100193 Institute of Medicinal Plant Development,Beijing Union Medical College & Chinese Academy of Medical Sciences,Beijing 100193,China 李勇,研究员,研究方向:药用植物病害生物防治关键技术;Tel:(010)57833360,E-Mail:liyong@implad.ac.cn 
基金项目:中医药行业科研专项(201407005);中国医学科学院医学与健康科技创新工程项目(2016-I2M-3-017)
中文摘要:目的:研究甲基营养型芽孢杆菌QM、BM2和HZ3不同抑菌活性部位。方法:采用对峙培养法及牛津杯验证提取物抑菌活性;菌丝形态用扫描电镜观察;菌株发酵上清液通过低温离心及微孔滤膜过滤制备;脂肽粗提物通过酸沉淀方法获得;有机溶剂萃取物分别用等量石油醚、乙酸乙酯、水饱和正丁醇萃取发酵上清液获得;蛋白粗提物通过硫酸铵沉淀结合透析法获得。结果:与三株甲基营养型芽孢杆菌对峙培养的人参致病菌菌丝生长均呈现畸形,甚至死亡;菌株发酵液和发酵上清液也有明显的抑菌活性,上清液中的脂肽粗提物、蛋白粗提物及水饱和正丁醇萃取物均对人参致病菌有不同程度的抑菌效果。结论:三株芽孢杆菌发酵液中含有多种抑菌活性物质,推测其可能单独或协同发挥抑菌作用。
中文关键词:人参  致病菌  甲基营养型芽孢杆菌  抑菌物质
 
Preliminary Analysis of Active Substances from Three Bacillus methylotrophicus Strains
Abstract:Objective:Study the antagonistic active sites of Bacillus methylotrophicus QM,BM2 and HZ3 strains.Methods:Confrontation culture and oxford cup were used to verify the antifungal activity of extracts.Mycelial morphology was observed under electron microscopy.Fermentation supernatant were prepared through low temperature centrifugation and microporous filter membrane filtration.The crude extract of lipoprotein is obtained by acid precipitation method.The organic solvent extraction was by extracted by isometric petroleum ether,ethyl acetate and water saturated n-butanol from the supernatant,respectively.Protein crude extract was obtained by ammonium sulfate precipitation and dialysis.Results:The mycelium growth of ginseng pathogens in confrontation with three strains of B.methylotrophicus shown abnormal growth and even death.The bacterial fermentation broth and supernatant also have significant antagonistic activity.The extracts of lipopeptide,protein crude extract and water saturated n-butanol extract in the supernatant have different inhibitory effects on ginseng pathogens.Conclusion:There are a variety of antibiotics in the fermentative of three strains of B.methylotrophicus,and it is proposed that these antibiotics may playing antagonistic activity alone or in synergy.
keywords:Panax ginseng  phytopathogens  Bacillus methylotrophicus  antibiotics
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