| AB-8大孔树脂分离纯化人参保健酒皂苷的工艺研究 |
| 投稿时间：2017-09-15 点此下载全文
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| 引用本文：诸晓波,李德坤,郭巧生,鞠爱春,叶正良.AB-8大孔树脂分离纯化人参保健酒皂苷的工艺研究[J].中国现代中药,2018,20(2):195-199 |
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| 中文摘要:目的：摸索AB-8大孔吸附树脂分离纯化人参保健酒中人参皂苷的工艺条件及参数。方法：以人参皂苷Rg1、Re、Rb1的含量为指标，考察大孔树脂在不同工艺条件下对人参单体皂苷的分离纯化能力。结果：AB-8大孔吸附树脂对皂苷具有较好的纯化富集性能。其最佳工艺条件为2.0 g树脂最大吸附1.0 g生药量，分别用10.0 mL纯化水、20.0 mL 0.05 moL·L-1氢氧化钠、30%甲醇除杂，90%甲醇洗脱，洗脱剂用量为20.0 mL，洗脱流速为0.6 mL·min-1。结论：该工艺简单易行，适用于高效液相测人参保健酒中单体皂苷含量。 |
| 中文关键词:保健酒 AB-8大孔吸附树脂 人参皂苷 高效液相色谱 |
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| Refining Technology of Ginseng Health Wine with AB-8 Macroporous Resin |
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| Abstract:Objective：The study aims at refining technology of ginseng health wine.Methods：Based on the content of ginsenoside Rg1，Re，Rb1，different types of refining and different technological conditions on the separation and purification capacity of single saponin in ginseng were compared.Results：AB-8 macroporous adsorption resin was the best choice to absorb and purify saponin among the methods.The best technological conditions were 90% methanol as elution agent，volume of elution agent was 20.0 mL，the flow eluting velocity was 0.6 mL·min-1.and 2.0 g resin absorb the 1.0 g material，0.05 moL·L-1 NaOH 30% methanol is the prefer concentration to wash the impurity.Conclusion：AB-8 macroporous adsorption resin is suitable to separate and purify single saponin in ginseng health wine. |
| keywords:Health wine AB-8 macroporous adsorption resin ginseng saponin HPLC |
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