不同浓缩方法对丹参提取液的影响
投稿时间:2017-06-08     点此下载全文
引用本文:刘铁军,孙胜斌,陈翔宇,王文鹏,周永妍,姜国志.不同浓缩方法对丹参提取液的影响[J].中国现代中药,2018,20(2):208-212
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作者中文名作者英文名单位中文名单位英文名E-Mail
刘铁军 LIU Tie-jun 河北省中药注射液工程技术研究中心,河北石家庄051430 Hebei Province Engineering Research Center for TCM Injection Shijiazhuang 051430,China  
孙胜斌 SUN Sheng-bin 神威药业集团有限公司,河北石家庄051430 Shineway Pharmaceutical Group Co.,Ltd.,Shijiazhuang 051430,China 孙胜斌,工程师,研究方向:药品工艺技术、质量研究及产业化研究,E-mail:sunshengbin2003@163.com 
陈翔宇 CHEN Xiang-yu 河北省中药注射液工程技术研究中心,河北石家庄051430 Hebei Province Engineering Research Center for TCM Injection Shijiazhuang 051430,China  
王文鹏 WANG Wen-peng 河北省中药注射液工程技术研究中心,河北石家庄051430 Hebei Province Engineering Research Center for TCM Injection Shijiazhuang 051430,China  
周永妍 ZHOU Yong-yan 神威药业集团有限公司,河北石家庄051430 Shineway Pharmaceutical Group Co.,Ltd.,Shijiazhuang 051430,China  
姜国志 JIANG Guo-zhi 中药注射剂新药技术开发国家地方联合工程实验室,河北石家庄051430 State-Local Joint Engineering Laboratory of New Drugs Development Technology for TCM Injection Shijiazhuang 051430,China  
基金项目:国家重大新药创制专项(2011ZX09401-306-004)
中文摘要:目的:研究不同浓缩方法对丹参提取液的影响。方法:以丹参提取液中丹参素钠、原儿茶醛、咖啡酸、迷迭香酸、丹酚酸B、总酚酸为指标成分,比较常压蒸发浓缩、减压浓缩、纳滤膜浓缩条件下各指标成分的变化情况。结果:常压浓缩、减压浓缩过程丹参素钠、原儿茶醛、咖啡酸、总酚酸含量逐步增加,增幅逐渐减小,常压浓缩含量增幅较减压浓缩大;丹酚酸B含量逐步减小,降幅逐渐减小,常压浓缩含量降幅较减压浓缩大;3种浓缩方法对迷迭香酸均无显著影响。膜浓缩过程对各含量均无显著影响。丹酚酸B降解符合一级反应的特点,常压浓缩降解回归方程为Ln(C)=-0.114 t+2.947 6,减压浓缩降解回归方程为Ln(C)=-0.083 t+2.947 6。结论:丹参提取液中存在热不稳定性成分,在受热条件下,其酚酸类成分会发生降解与转化,生产过程中应严格控制温度、真空度、时间等参数,以保证批间的一致稳定,使用纳滤膜浓缩方法可最大程度地保持与药液物质基础一致。应采用纳滤膜浓缩方法对丹参提取液进行浓缩。
中文关键词:丹参提取液  浓缩  有效成分
 
Effect of Different Concentration Method on Salvia miltiorrhiza
Abstract:Objective:To investigate effect of different concentration method on extraction of Salvia miltiorrhiza.Methods:Sodium danshensu,protocatechuic aldehyde,caffeic acid,rosmarinic acid,salvianolic acid B and total phenolic acid were used as marker compounds,the changes of the indexes under the atmospheric concentration,decompress concentration and nanofiltration membrane concentration were compared.Results:The results indicated that the contents of sodium danshensu,protocatechuic aldehyde,caffeic acid and total phenolic acid increased gradually,and the increase gradually slowed down under the atmospheric concentration and decompress concentration,the increasing range was larger in atmospheric concentration.The contents of salvianolic acid B decreased gradually,and the decline gradually decreased,the decreasing range was larger in atmospheric concentration.All the three concentration methods showed no significant difference on the contents of rosmarinic acid.There was no significant difference under the nanofiltration membrane concentration.It shows that the degradation of salvianolic acid B can be expressed as a first order reaction,the degradation regression equation of decompress concentration was Ln(C)=-0.083 t+2.947 6,the degradation regression equation of atmospheric concentration was Ln(C)=-0.114 t+2.947 6.Conclusion:Some compounds of S.miltiorrhiza is thermally unstable,as phenolic acid will degrade under heating conditions.To ensure uniformly stability between different batches of S.miltiorrhiza,parameters should be strictly controlled,such as the heating temperature,time and vacuum degree in the concentrating process.The concentration of nanofiltration membrane have the advantages,the substances are reserved in maximum.The extraction solution of S.miltiorrhiza should be concentrated by nanofiltration membrane enrichment method.
keywords:Salvia miltiorrhiza extract  enrichment  active ingredients
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