| DNA条形码技术在新疆进口民族民间药材基原考证中的应用 |
| 投稿时间：2017-12-08 点此下载全文
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| 引用本文：李亚伟,刘强,樊丛照,李晓瑾,刘富铭.DNA条形码技术在新疆进口民族民间药材基原考证中的应用[J].中国现代中药,2018,20(4):377-381 |
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| 基金项目:国家质量监督检验检疫总局科技计划项目（2016IK262）；乌鲁木齐市科技局项目（G151010003） |
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| 中文摘要:目的：采用DNA条形码技术，研究探索新疆进口民族民间药材基原考证。方法：使用试剂盒法提取药材总DNA，采用ITS2序列、psbA-trnH序列对30份进口维吾尔药材样品进行PCR扩增并双向测序，利用MEGA7.0等软件分析序列信息，将序列在DNA条形码基原植物鉴定数据库及GenBank数据库中进行比对，搜索相似性最高的植物，判定药材可能的基原。结果：综合ITS2及psbA-trnH 实验结果，30份药材中有28份样品测序成功并得到有效的鉴定序列，相似性对比结果显示有15种药材与文献记载基原植物一致，7份与基原植物为近缘种，6种药材与文献记载基原植物不同属。结论：进口维吾尔药材的基原调查与考证工作迫在眉睫。 |
| 中文关键词:进口药材 DNA条形码 ITS2 psbA-trnH 基原植物 |
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| Application of DNA Barcoding Technology in Studying the Original Species of Imported Ethnical Medicinal Materials in Xinjiang |
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| Abstract:Objective：To explore the validity of using DNA barcoding technology to identify the original species of imported ethnical medicinal materials in Xinjiang.Methods：Total DNA was extracted by DNA extraction kit from thirty samples，ITS2 and psbA-trnH sequences were amplified by PCR methord and bidirectional sequenced.Sequence informations were analysed by MEGA7.0 software，all ITS2 and psbA-trnH sequences were identified by comparison of sequence similarity in professional nucleic acid sequence database，the most similar plant sequence were searched to determine the original species.Results：According to ITS2 and psbA-trnH sequences，twenty-eight medicinal samples obtained effective sequences.The comparison of sequence similarity results showed that fifteen kinds of medicinal materials were consistent with the original species as recorded in the literature，seven samples were closely related to their original species，and six samples were different with the original species as recorded.Conclusion：It is urgent to investigate and study the original species of imported Uygur medicinal materials. |
| keywords:Imported medicinal materials DNA barcode ITS2 psbA-trnH original species |
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