荒漠肉苁蓉中苯乙醇苷类与多糖含量的测定及抗氧化作用研究
投稿时间:2017-12-03     点此下载全文
引用本文:覃文婷,王小明,徐荣,宿美凤,雒晓梅,常晓燕,陈君,石钺.荒漠肉苁蓉中苯乙醇苷类与多糖含量的测定及抗氧化作用研究[J].中国现代中药,2018,20(4):426-431
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作者中文名作者英文名单位中文名单位英文名E-Mail
覃文婷 TAN Wen-ting 北京中医药大学 中药学院,北京100029 College of Traditional Chinese Medicine,Beijing University of Chinese Medicine,Beijing 100029,China  
王小明 WANG Xiao-ming 中国医学科学院中国协和医科大学 药用植物研究所,北京100193 The Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100193,China  
徐荣 XU Rong 中国医学科学院中国协和医科大学 药用植物研究所,北京100193 The Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100193,China  
宿美凤 SU Mei-feng 北京中医药大学 中药学院,北京100029 College of Traditional Chinese Medicine,Beijing University of Chinese Medicine,Beijing 100029,China  
雒晓梅 LUO Xiao-mei 中国医学科学院中国协和医科大学 药用植物研究所,北京100193 The Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100193,China  
常晓燕 CHANG Xiao-yan 中国医学科学院中国协和医科大学 药用植物研究所,北京100193 The Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100193,China  
陈君 CHEN Jun 中国医学科学院中国协和医科大学 药用植物研究所,北京100193 The Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100193,China  
石钺 SHI Yue 中国医学科学院中国协和医科大学 药用植物研究所,北京100193 The Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100193,China 石钺,研究员,研究方向:中药及复方药效物质基础及其质量控制方法研究;E-mail:shiyue1029@126.com 
基金项目:国家自然基金资助项目(31570344)
中文摘要:目的:同时测定荒漠肉苁蓉中5种苯乙醇苷类与多糖含量及其抗氧化活性。方法:制备荒漠肉苁蓉总苷与70%醇提物,建立UPLC色谱条件,测定其中5种苯乙醇苷类成分含量,苯酚硫酸法测定多糖含量,同时运用体外清除DPPH自由基实验评价三者抗氧化活性。结果:荒漠肉苁蓉中含量最高成分为松果菊苷,其次为毛蕊花糖苷,醇提物中两者含量为1.9%,5种苯乙醇苷类成分总含量为2%;醇提物经纯化后,两者含量达到17%,5种苯乙醇苷类成分总量达到20.2%,含量提高约9~10倍;苯酚硫酸法测得多糖含量为86.87%;体外清除·DPPH实验中,以IC50值为评价标准,其清除DPPH自由基能力大小为:总苷>70%醇提物>多糖。结论:文章首次同时检测荒漠肉苁蓉中苯乙醇苷类与多糖组份的含量,且苯乙醇苷类成分抗氧化活性显著高于多糖,为荒漠肉苁蓉质量评价、活性成分的利用提供数据参考。
中文关键词:荒漠肉苁蓉  超高效液相色谱  苯酚硫酸法  苯乙醇苷类  多糖  抗氧化
 
Content Determinating of Phenylethanoid Glycosides and Polysaccharides in Cistanche deserticola and Their Antioxidant Activity
Abstract:Objective:To determine the content of 5 kinds of phenylethanoid glycosides(PhG)and polysaccharides in Cistanche deserticola and their antioxidant activity simultaneously.Methods:The PhGs and ethanol extract were prepared,the content of 5 PhGs was determined by UPLC,the content of polysaccharides was detected by phenol-sulfuric acid,scavenging ·DPPH test in vitro to evaluate their antioxidant activity;Results:The highest content of C.deserticola was echinacoside,followed by acteoside,the content of echinacoside and acteoside was 1.9% in ethanol extract and the total content of five PhGs was 2%;After purified,the content of echinacoside and acteoside reached 17%,the total content of 5 PhGs reached 20.2%.The content of polysaccharides was 86.87%,in the scavenging ·DPPH test,based on IC50value,3 system scavenge ability was:PhGs>ethanol extract>polysaccharides. Conclusion:The content of PhGs and polysaccharides in C.deserticola was firstly detected simultaneously.The antioxidant activity of PhGs was significantly higher than that of polysaccharides.The results provide data for quality evaluation and utilization of active ingredients in C.deserticola .
keywords:Cistanche deserticola  UPLC  phenol-sulfuric acid  phenylethanoid glycosides  polysaccharides  antioxidant activity
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