Content Determinating of Phenylethanoid Glycosides and Polysaccharides in Cistanche deserticola and Their Antioxidant Activity
Abstract:Objective:To determine the content of 5 kinds of phenylethanoid glycosides(PhG)and polysaccharides in Cistanche deserticola and their antioxidant activity simultaneously.Methods:The PhGs and ethanol extract were prepared,the content of 5 PhGs was determined by UPLC,the content of polysaccharides was detected by phenol-sulfuric acid,scavenging ·DPPH test in vitro to evaluate their antioxidant activity;Results:The highest content of C.deserticola was echinacoside,followed by acteoside,the content of echinacoside and acteoside was 1.9% in ethanol extract and the total content of five PhGs was 2%;After purified,the content of echinacoside and acteoside reached 17%,the total content of 5 PhGs reached 20.2%.The content of polysaccharides was 86.87%,in the scavenging ·DPPH test,based on IC50value,3 system scavenge ability was:PhGs>ethanol extract>polysaccharides. Conclusion:The content of PhGs and polysaccharides in C.deserticola was firstly detected simultaneously.The antioxidant activity of PhGs was significantly higher than that of polysaccharides.The results provide data for quality evaluation and utilization of active ingredients in C.deserticola .