| 银翘散水提物主要化学成分峰的来源归属研究 |
| 投稿时间：2017-09-04 点此下载全文
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| 引用本文：宋健,张会敏.银翘散水提物主要化学成分峰的来源归属研究[J].中国现代中药,2018,20(4):458-461 |
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| 基金项目:山东省自然科学基金项目（ZR2014HQ046）；山东省中医药科技发展计划项目（2015-175） |
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| 中文摘要:目的：通过建立银翘散及其各单味药水提物的UPLC指纹图谱，对银翘散水提物中各主要化学成分峰进行对比分析及归属，为进一步阐明银翘散物质基础及诠释组方配伍原理奠定基础。方法：采用UltiMate 3000 XRS UPLC，Thermo（250 mm×4.6 mm，5 μm）C18柱；色谱条件：甲醇-0.05%磷酸水为流动相梯度洗脱；柱温：30 ℃；检测波长237 nm；流速：1 mL·min-1；分析时间：100 min。建立了银翘散及其各单味药水提物进行UPLC指纹图谱，并利用指纹图谱处理软件对银翘散及其各单味药UPLC指纹图谱进行数据分析。结果：在银翘散水提物的34个主要成分峰中，其中18个成分峰来源于君药金银花，8个成分峰来源于君药连翘，5个成分峰来源于薄荷，各有4个成分峰来源于荆芥、牛蒡子、竹叶，3个成分峰来源于甘草，未见来源于淡豆豉、芦根、桔梗3味药的成分峰。并归属了绿原酸、连翘苷、牛蒡子苷等7个成分峰。结论：有助于阐释银翘散物质基础及组方配伍原理。 |
| 中文关键词:银翘散 水提物 指纹图谱 |
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| Study on Main Components in Aqueous Extract of Yinqiao-San |
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| Abstract:Objective：By establishing the UPLC fingerprint of Yinqiao-San and its individual extracts，the main chemical composition peaks were compared and analyzed，which could lay a foundation for further clarifying the material basis and interpreting the principle of compatibility of Yinqiao-San.Methods：The UltiMate 3000 XRS UPLC and Thermo（250 mm×4.6 mm，5 μm）C18 column were used.The chromatographic conditions were：the mobile phase gradient elution was methanol-0.05% phosphoric acid，the column temperature was 30 ℃，the detection wavelength was 237 nm，the flow rate was 1 mL·min-1and the analysis time was 100 min.Then，the aqueous extracts UPLC fingerprints of Yinqiao-San and all single medicines were established，which were analyzed by the fingerprint processing software.Results：In the 34 main component peaks of aqueous extract of Yinqiao-San，there were 18 component peaks from Lonicerae Flos，8 component peaks from Forsythiae Fructus，5 component peaks from Menthae Haplocalycis Herba.And there were 4 component peaks from each of Schizonepetae Herba，Arctii Fructus and Bambosae Folia.There were only 3 component peaks from Glycyrrhizae Radix et Rhizoma.There were no component peaks from Sojae Semen Praeparatum，Phragmitis Rhizoma and Platycodonis Radix.7 component peaks were belonged.Conclusion：The results help to explain the material basis and compatibility principle of Yinqiao-San. |
| keywords:Yinqiao-San aqueous extract fingerprint |
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