桃花中绿原酸抗氧化活性的研究
投稿时间:2018-01-31     点此下载全文
引用本文:梁萱,李嘉会,梁永锋.桃花中绿原酸抗氧化活性的研究[J].中国现代中药,2018,20(6):697-701
DOI:
摘要点击次数: 727
全文下载次数: 427
        
作者中文名作者英文名单位中文名单位英文名E-Mail
梁萱 LIANG Xua 兰州军区兰州总医院 药剂科,甘肃兰州730000 Pharmacy deportment,Lanzhou General Hospital of Lanzhou Military Region,Lanzhou 730000,China  
李嘉会 LI Jia-Hui 宁夏师范学院 化学化工学院,宁夏固原756000 School of Chemistry and Chemical Engineering,Ningxia Normal University,Guyuan 756000,China  
梁永锋 LIANG Yong-feng 宁夏师范学院 化学化工学院,宁夏固原756000 School of Chemistry and Chemical Engineering,Ningxia Normal University,Guyuan 756000,China 梁永锋,教授,研究方向:天然产物分析及应用研究;E-mail:qylyf338@163.com 
基金项目:宁夏回族自治区研究生教育创新计划项目(YXW2017019)
中文摘要:以桃花提取物为试材,维生素C和绿原酸标准品为对照品,采用K3\[Fe(CN)6\]测定其还原能力,D-脱氧核糖-铁体系法、二苯代苦味酰基自由基(DPPH·)和邻苯三酚自氧化法测定桃花提取物中绿原酸的抗氧化活性。结果表明:桃花提取物具有较强的还原性,其抗氧化活性与绿原酸含量密切相关。其中桃花提取物抗羟基自由基活性高于Vc,浓度为200 μ·mL-1时,可达到66.42%;抗超氧阴离子自由基活性略低于Vc,浓度为200 μg·mL-1时,抗氧化活性为95.78%;抗DPPH·自由基的活性比Vc略高,当浓度为200 μg·mL-1时,抗氧化活性为82.12%。 该研究说明,桃花提取物的还原性和抗氧化活性与绿原酸具有良好的效量关系,桃花中的抗氧化活性物质主要是绿原酸。
中文关键词:桃花提取物  抗氧化活性  绿原酸  相关性
 
Antioxidant Activity of Chlorogenic Acid Extracted from Peach Blossom
Abstract:Taking Peach blossom as test material,antioxidant activity of chlorgenic acid extracted from peach blossom was measured by D-deoxyribose-iron system method along with 2,2-diphenyl-1-picrylhydrazyl pyrogallol autoxidation method,while vitamin C and chlorgenic acid were used as reference substance and reduction ability of the extract was measured by using K3\[Fe(CN)6\].The results showed that peach blossom extract had strong reducibility,and its antioxidant activity was closely related to chlorgenic acid content.The extract had higher anti-hydroxyl and anti-DPPHo activity but lower anti-superoxide anion activity than vitamin C.Its reducibility with concentration of 200 μg·mL-1 for hydroxyl was as high as 66.42%,and antioxidant activity was 95.78% and 82.12% for superoxide anion and DPPHo,respectively.This study showed that the main extract of peach blossom was chlorgenic acid and its concentration largely determine reductive and antioxidant activities for the extract.
keywords:Peach blossom extraction  antioxidant activity  chlorogenic acid  relevance
查看全文   查看/发表评论  下载PDF阅读器