桃花中绿原酸抗氧化活性的研究 |
投稿时间:2018-01-31 点此下载全文
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引用本文:梁萱,李嘉会,梁永锋.桃花中绿原酸抗氧化活性的研究[J].中国现代中药,2018,20(6):697-701 |
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基金项目:宁夏回族自治区研究生教育创新计划项目(YXW2017019) |
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中文摘要:以桃花提取物为试材,维生素C和绿原酸标准品为对照品,采用K3\[Fe(CN)6\]测定其还原能力,D-脱氧核糖-铁体系法、二苯代苦味酰基自由基(DPPH·)和邻苯三酚自氧化法测定桃花提取物中绿原酸的抗氧化活性。结果表明:桃花提取物具有较强的还原性,其抗氧化活性与绿原酸含量密切相关。其中桃花提取物抗羟基自由基活性高于Vc,浓度为200 μ·mL-1时,可达到66.42%;抗超氧阴离子自由基活性略低于Vc,浓度为200 μg·mL-1时,抗氧化活性为95.78%;抗DPPH·自由基的活性比Vc略高,当浓度为200 μg·mL-1时,抗氧化活性为82.12%。 该研究说明,桃花提取物的还原性和抗氧化活性与绿原酸具有良好的效量关系,桃花中的抗氧化活性物质主要是绿原酸。 |
中文关键词:桃花提取物 抗氧化活性 绿原酸 相关性 |
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Antioxidant Activity of Chlorogenic Acid Extracted from Peach Blossom |
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Abstract:Taking Peach blossom as test material,antioxidant activity of chlorgenic acid extracted from peach blossom was measured by D-deoxyribose-iron system method along with 2,2-diphenyl-1-picrylhydrazyl pyrogallol autoxidation method,while vitamin C and chlorgenic acid were used as reference substance and reduction ability of the extract was measured by using K3\[Fe(CN)6\].The results showed that peach blossom extract had strong reducibility,and its antioxidant activity was closely related to chlorgenic acid content.The extract had higher anti-hydroxyl and anti-DPPHo activity but lower anti-superoxide anion activity than vitamin C.Its reducibility with concentration of 200 μg·mL-1 for hydroxyl was as high as 66.42%,and antioxidant activity was 95.78% and 82.12% for superoxide anion and DPPHo,respectively.This study showed that the main extract of peach blossom was chlorgenic acid and its concentration largely determine reductive and antioxidant activities for the extract. |
keywords:Peach blossom extraction antioxidant activity chlorogenic acid relevance |
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