Determination of Various Endogenous Hormones in Flower Buds of Panax ginseng by UPLC-MS/MS
Abstract:Objective:To explore the important regulation role of plant endogenous hormones on secondary metabolism of Chinese herbal medicine. Methods:Five kinds of endogenous phytohormones(auxin,gibberellin,abscisic acid,jasmonic acid,salicylic acid)in flowers of Panax ginseng were measured by UPLC-MS/MS.Results:The method for determination of 5 endogenous phytohormone content in P.ginseng all has high specificity.The higher content of ABA and SA(plant resistance related)was 1 126.17±232.52 ng·g-1 and 3 929.43±898.52 ng·g-1 respectively;The lower content of IBA and GA3(plant growth related)was 516.70±138.55 ng·g-1 and 692.33±228.50 ng·g-1 respectively;In jasmonic acid synthesis pathway,the highest content of jasmonate precursor,OPDA,was 47 158.84 + 12 910.55 ng·g-1.JA was not detected and JA-ile was 22.08+6.27 ng·g-1. Conclusion:The study explored the distribution of 5 endogenous phytohormones in ginseng flowers,which laid the foundation for further study on the regulation of endogenous phytohormones on ginsenoside biosynthesis.