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作者中文名	作者英文名	单位中文名	单位英文名	E-Mail
任乔森	REN Qiao-sen	吉林省药品检验所，吉林长春130033	Jilin Institute for Drug Control，Changchun 130033，China
王海洋	WANG Hai-yang	吉林省药品检验所，吉林长春130033	Jilin Institute for Drug Control，Changchun 130033，China
翟宏宇	ZHAI Hong-yu	吉林省药品检验所，吉林长春130033	Jilin Institute for Drug Control，Changchun 130033，China
赵琳琳	ZHAO Lin-lin	吉林省药品检验所，吉林长春130033	Jilin Institute for Drug Control，Changchun 130033，China
李秀芬	LI Xiu-fen	吉林省药品检验所，吉林长春130033	Jilin Institute for Drug Control，Changchun 130033，China	李秀芬，主任药师；研究方向：药品质量标准研究；Tel：（0431）892831011，E-mail：lixiufen-2008@163.com
唐秋竹	TANG Qiu-zhu	吉林省药品检验所，吉林长春130033	Jilin Institute for Drug Control，Changchun 130033，China
赵琪钟	ZHAO Qi-zhong	普洱市食品药品检验检测中心，云南普洱665000	Puer Institute for food and Drug Control，Puer 665000，China

中文摘要:目的：建立朝鲜白头翁的特征图谱，为科学有效评价和控制其质量提供可靠依据。方法：采用高效液相色谱法，Thermo Hypersil BDS C18色谱柱（4.6×250 mm，5 μm），以乙腈-水作为流动相进行梯度洗脱，流速0.8 mL·min-1，柱温35 ℃，检测波长201 nm。结果：初步建立了朝鲜白头翁的特征图谱，标定了20个共有峰，并指认出2个。结论：采用HPLC法建立朝鲜白头翁的特征图谱，操作简单，选择性高，分离效果好，可用于朝鲜白头翁及其混淆品的鉴别和质量控制。

中文关键词:朝鲜白头翁高效液相色谱特征图谱质量控制

Study on HPLC Characteristic Chromatogram of Pulsatilla cernua

Abstract:Objective：To establish HPLC characteristic chromatograms of Pulsatilla cernua，so as to provide a reliable method for scientific evaluation and effective quality control of medicinal material.Methods：The effective components were separated by HPLC with Thermo Hypersil BDS C18（4.6×250 mm，5 μm）in a gradient elution of acetonitrile and water as the mobile phase at the flow rate of 0.8 mL·min-1 and temperature of 35 ℃ and detection wavelength of 201 nm.Results：The specific chromatogram of Pulsatilla cernua was established and 20 common peaks were indicated and 2 peaks were indicated.Conclusion：The HPLC method was precise，stable and reproducible.These established methods are practical and suitable for the identify and quality control of Pulsatilla cernua.

keywords:Pulsatilla cernua HPLC characteristic chromatograms quality control

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