| 储藏条件对柏子仁污染黄曲霉毒素的影响及脱除方法探讨 |
| 投稿时间：2018-03-16 点此下载全文
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| 引用本文：陈建茹,董伟伟,彭娟,焦晓林,吴馨琰,高微微.储藏条件对柏子仁污染黄曲霉毒素的影响及脱除方法探讨[J].中国现代中药,2018,20(6):725-728 |
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| 基金项目:国家中药标准化项目（ZYBZH-Y-JIN-34） |
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| 中文摘要:目的：了解不同储藏条件对柏子仁药材黄曲霉毒素污染的影响，并探讨洗脱方法对柏子仁中黄曲霉毒素的脱除效果，为确定柏子仁药材的合理储藏条件及脱毒技术提供参考。方法：HPLC方法测定10个批次柏子仁黄曲霉毒素的污染程度，并设计2个温度及2种包装进行储藏试验，对比不同储藏条件下柏子仁黄曲霉毒素B1（AFB1）及黄曲霉毒素B1、B2、G1、G2之和（AFs）的污染量。采用乙醇洗、水洗两种方法对柏子仁中黄曲霉毒素进行脱除。结果：有9个批次柏子仁存在黄曲霉毒素污染，其中5个批次的污染程度超出2015版《中国药典》限量标准。在10 ℃和25 ℃两个储藏温度下，AFB1和AFs均随储藏时间的延长而逐渐增加，储藏2个月时，AFB1的最大增加量达到起始的8.3倍，AFs的最大增加量达到7.8倍。真空包装与普通塑料袋相比，并不能显著降低毒素的增加量。乙醇洗对柏子仁中AFB1和AFs脱除率达到90%以上，水洗可以达到87%以上。结论：柏子仁在10 ℃、25 ℃时采用塑料袋及真空包装储藏期不宜超过1个月，两种清洗方法可部分脱除柏子仁中黄曲霉毒素。 |
| 中文关键词:柏子仁 黄曲霉毒素 储藏温度 包装 脱毒技术 |
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| Effects of Storage Conditions on Aflatoxin Contamination of Platycladi Seed and Detoxification Methods |
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| Abstract:Objective：In order to understand the effects of different storage conditions on aflatoxin contamination of platycladi seed and the detoxification effect of different washing methods，provide references for developing reasonable storage conditions and detoxification techniques for platycladi seed. Methods：The contents of aflatoxins on 10 batches of platycladi seed were detected by HPLC.The storage experiment of platycladi seed was designed for two temperatures and two packages，and the content of aflatoxin B1（AFB1）and aflatoxin B1+B2+G1+G2（AFs）were analyzed after storage.Platycladi seed were washed by ethanol or water to remove the contaminated aflatoxin. Results：Aflatoxins were found on 9 batches of platycladi seed，of which 5 batches exceeded the maximum level permitted by 2015 Chinese pharmacopoeia.In storage experiment，AFB1 and AFs contaminated on platycladi seed were increased with time at 10 ℃ and 25 ℃.After 2 months of storage，AFB1 was increased up to 8.3 times than pre-storage，while AFs was increased up to 7.8 times.Compared with plastic bags package，aflatoxin contaminations on platycladi seed were not decreased obviously in vacuum package.The removal rates of AFB1 and AFs on platycladi seed were over 90% after ethanol washing，while they were over 87% when washed by water. Conclusion：The storage period of platycladi seed in plastic bag and vacuum package should not exceed 1 month at 10 ℃ and 25 ℃.Water washing and ethanol washing could partially remove aflatoxins from platycladi seed. |
| keywords:Platycladi seed aflatoxin storage temperature package detoxification technique |
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