| 响应面法优化柴胡多糖提取工艺 |
| 投稿时间：2017-12-15 点此下载全文
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| 引用本文：邓寒霜,杨丽娜.响应面法优化柴胡多糖提取工艺[J].中国现代中药,2018,20(6):742-747 |
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| 基金项目:陕西欧珂药业有限公司专项资助科研项目（2016HXKY011） |
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| 中文摘要:目的：优化柴胡多糖提取工艺。方法：采用苯酚-硫酸显色，紫外可见分光光度法（UV）在490 nm波长处测定柴胡多糖的吸光度，计算多糖提取率并以此为评价指标，在单因素试验的基础之上，选取提取温度、水料比、提取时间为考察因素，利用Box-Behnken方法进行三因素三水平试验设计。结果：优选出柴胡多糖的最佳提取工艺参数为提取温度95 ℃、水料比45∶1、提取时间1.5 h，在上述条件下重复提取2次；得多糖提取率与各试验变量间的回归方程为Y=18.99+1.20A+0.39B+0.072C-0.16AB-0.22AC-0.20BC-1.22A2-0.35B2-0.14C2，r=0.983 6。结论：经验证在优选条件下柴胡多糖提取率为19.16%，与预测值19.39%相近，说明采用该工艺条件提取柴胡多糖效果较好，试验所得回归方程可准确预测结果。 |
| 中文关键词:柴胡多糖 提取率 苯酚-硫酸法 响应面分析法 |
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| Optimization of Polysaccharide Extraction from Bupleuri Radix Using Response Surface Methodology |
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| Abstract:Objective：To optimize the extraction technology of Bupleurum polysaccharide.Methods：Bupleurum polysaccharide was determined by UV and phenol-sulfuric acid method at 490 nm，the extraction rate of Bupleurum polysaccharide was calculated.On the basis of single-factor experiments，choosing extraction temperature，water to material ratio and extraction time as independent variable，the extraction process of Bupleurum polysaccharide was optimized by using response surface methodology with three factors and three levels.Results：The optimum extraction parameters of Bupleurum polysaccharides were as follows：95 ℃ of the extraction temperature，45∶1 of water to material ratio，1.5 h of extraction time.The regression equation between the extraction rate of polysaccharides and the experimental variables was Y=18.99+1.20A+0.39B+0.072C-0.16AB-0.22AC-0.20BC-1.22A2-0.35B2-0.14C2，r=0.983 6.Conclusion：Under the optimum conditions，the extraction rate of Bupleurum polysaccharide was 19.16%，which was similar to the predicted value of 19.39%.The results showed that the extraction process of Bupleurum polysaccharide was better，and the regression equation could predict the result accurately. |
| keywords:Bupleurum polysaccharide extraction rate phenol sulfuric acid method response surface methodology |
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