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作者中文名	作者英文名	单位中文名	单位英文名	E-Mail
李婷	LI Ting	山东中医药大学药学院，山东济南250355	Shandong University of Traditional Chinese Medicine，College of Pharmacy，Jinan 250355，China
于海涛	YU Hai-tao	山东中医药大学药学院，山东济南250355	Shandong University of Traditional Chinese Medicine，College of Pharmacy，Jinan 250355，China
金玉翠	JIN Yu-cui	山东中医药大学药学院，山东济南250355	Shandong University of Traditional Chinese Medicine，College of Pharmacy，Jinan 250355，China
刘晓美	LIU Xiao-mei	山东中医药大学药学院，山东济南250355	Shandong University of Traditional Chinese Medicine，College of Pharmacy，Jinan 250355，China
李圆圆	LI Yuan-yuan	山东中医药大学药学院，山东济南250355	Shandong University of Traditional Chinese Medicine，College of Pharmacy，Jinan 250355，China
王常瞵	WANG Chang-lin	山东中医药大学药学院，山东济南250355	Shandong University of Traditional Chinese Medicine，College of Pharmacy，Jinan 250355，China
吴明泽	WU Ming-ze	山东中医药大学药学院，山东济南250355	Shandong University of Traditional Chinese Medicine，College of Pharmacy，Jinan 250355，China
代龙	DAI Long	山东中医药大学药学院，山东济南250355	Shandong University of Traditional Chinese Medicine，College of Pharmacy，Jinan 250355，China
高鹏	GAO Peng	山东中医药大学药学院，山东济南250355	Shandong University of Traditional Chinese Medicine，College of Pharmacy，Jinan 250355，China	高鹏，副教授，研究方向：中药制剂新技术、新剂型；E-mail：48395504@qq.com

基金项目:山东省重点研发计划项目（2015GSF119012）

中文摘要:目的：研究不同分子量猪皮胶原肽的抗氧化特性，筛选出抗氧化活性较强的组分。方法：采用仿生酶解技术酶解新鲜猪皮得到酶解原液，酶解原液经超滤得到M＜1 kDa、1 kDa＜M＜3 kDa、M＞3 kDa 3个不同分子量肽段，猪皮经水提取得到猪皮水提液，上述5组分分别冻干，得到冻干粉。对不同分子量范围的猪皮胶原肽5组分进行肽含量测定和抗氧化性研究，包括对DPPH·自由基、O2-·自由基及·OH自由基的清除实验。结果：各组分的肽含量分别为酶解原液组分64.62%、1～3 kDa组分62.63%、大于3 kDa组分62.13%、水提液组分60.57%、小于1 kDa组分59.75%。各组分清除DPPH·自由基能力顺序为：小于1 kDa组分＞1～3kDa组分＞酶解原液组分＞水提液组分＞大于3 kDa组分；各组分清除O2-·自由基能力顺序为：小于1 kDa组分＞酶解原液组分＞1～3 kDa组分＞大于3 kDa组分＞水提液组分；各组分清除·OH自由基能力顺序为：小于1 kDa组分＞1～3 kDa组分＞酶解原液组分＞大于3 kDa组分＞水提液组分。结论：不同分子量的猪皮胶原肽组分均有一定的抗氧化活性，其中M＜1 kDa组分具有较强的自由基清除能力。

中文关键词:猪皮胶原肽抗氧化自由基清除

Study on Antioxidative Effect of Porcine Collagen Peptide

Abstract:Objective：To study the antioxidative properties of collagen peptides with different molecular weights and screen out the components with the highest antioxidative activity.Methods：Fresh pigskin was digested by biomimetic enzymolysis to obtain the enzymatic hydrolyzate solution.Three different molecular weight peptides of M<1 kDa，1 kDa3 kDa were obtained by ultrafiltration.The pigskin was extracted by water pig skin water extract，the above five components were lyophilized to obtain lyophilized powder.The peptide content and antioxidative activity of porcine skin peptide 5 components in different molecular weight range were studied，including the scavenging experiments of DPPH·free radical，O2-· radical and ·OH radical.Results：the peptide content of each component was the original enzyme solution：64.62%，1-3 kDa component：62.63%，than 3kDa component：62.13%，water extract component：60.57%，less than the 1 kDa component：59.75%；The order of scavenging DPPH·free radical of each component was：1 kDa component> 1 -3 kDa component> enzymatic hydrolyzate component> aqueous extract component> more than 3kDa component；The order of scavenging O2-·free radical capacity was：less than 1 kDa component> digestive juice component> 1 -3 kDa component> more than 3 kDa component> water extract component；ability to eliminate·OH radical of each component in order of：less than 1 kDa component > 1-3 kDa component> enzymatic solution component> more than 3 kDa component> aqueous extract component.Conclusion：The porcine skin peptides with different molecular weights all have certain antioxidant activity，and M <1 kDa has strong free radical scavenging ability.

keywords:Porcine collagen peptide antioxidant free radical scavenging

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