HPLC定量分析千斤拔药材中染料木苷和染料木素的含量
投稿时间:2018-03-15     点此下载全文
引用本文:张鹏,彭开锋,李伏君,龚云,刘逆夫,付卡利,杨秀伟,王龙.HPLC定量分析千斤拔药材中染料木苷和染料木素的含量[J].中国现代中药,2018,20(8):948-952
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作者中文名作者英文名单位中文名单位英文名E-Mail
张鹏 ZHANG Peng 株洲千金药业股份有限公司,湖南株洲412000 Zhuzhou Qianjin Pharmaceutical Co.,Ltd.,Zhuzhou 412003,China  
彭开锋 PENG Kai-feng 株洲千金药业股份有限公司,湖南株洲412000 Zhuzhou Qianjin Pharmaceutical Co.,Ltd.,Zhuzhou 412003,China  
李伏君 LI Fu-jun 株洲千金药业股份有限公司,湖南株洲412000 Zhuzhou Qianjin Pharmaceutical Co.,Ltd.,Zhuzhou 412003,China  
龚云 GONG Yun 株洲千金药业股份有限公司,湖南株洲412000 Zhuzhou Qianjin Pharmaceutical Co.,Ltd.,Zhuzhou 412003,China  
刘逆夫 LIU Ni-fu 株洲千金药业股份有限公司,湖南株洲412000 Zhuzhou Qianjin Pharmaceutical Co.,Ltd.,Zhuzhou 412003,China  
付卡利 FU Ka-li 株洲千金药业股份有限公司,湖南株洲412000 Zhuzhou Qianjin Pharmaceutical Co.,Ltd.,Zhuzhou 412003,China  
杨秀伟 YANG Xiu-wei 北京大学 药学院 天然药物学系/天然药物及仿生药物国家重点实验室,北京100191 Department of Natural Medicines,State Key Laboratory of Natural and Biomimetic Drugs,School of Pharmaceutical Sciences,Peking University,Beijing 100191,China 杨秀伟,教授,博士生导师,研究方向:天然产物化学与药物代谢;Tel:(010)82801569,E-mail:xwyang@bjmu.edu.cn 
王龙 WANG Long 约翰霍普金斯大学,美国巴尔的摩21218 Johns Hopkins University,Baltimore 21218,America  
基金项目:国家中药标准化项目(2YB2H-C-HUN-21)
中文摘要:目的:建立千斤拔药材的质量控制方法。方法:采用高效液相色谱法测定其中的染料木苷和染料木素的含量;色谱柱为Kromasil 100-5 C18(250 mm×4.6 mm,5 μm),梯度洗脱,流动相为乙腈-0.1%磷酸水溶液,流速为1 mL·min-1,检测波长为260 nm。结果:染料木苷和染料木素分别在质量浓度17.483~279.720 μg·mL-1和4.088~65.406 μg·mL-1与峰面积线性关系良好。平均回收率,染料木苷为104.27%,RSD为2.61%(n=9);染料木素为97.16%,RSD为5.54%(n=9)。不同产地千斤拔药材中染料木苷和染料木素的含量有明显差异。结论:建立的含量测定方法操作简便、分离效果好、准确,能满足千斤拔药材中染料木苷和染料木素含量的准确测定,为千斤拔药材的质量评价以及合理开发利用提供了科学依据。
中文关键词:千斤拔  染料木苷  染料木素  高效液相色谱  质量标准
 
Quantitative Determination of Genistin and Genistein in Moghania Medicinal Material by HPLC
Abstract:Objective:To establish a method for the quality control of Moghania medicinal material.Methods:An HPLC method for quantitative analysis of genistin and genistein in the Moghania medicinal material,including the roots of Moghania macrophylla(Willd.) O.Kuntze and M.philippinensis(Merr.et Rolfe) Li,was carried out.The method was used by HPLC with Kromasil 100-5 C18 column(250 mm×4.6 mm,5 μm) using gradient elution with acetonitrile and 0.1% aqueous phosphoric acid as the mobile phase at a flow rate of 1 mL·min-1,and the detection wavelength was 260 nm.Results:Genistin and genistein showed good linearity in the ranges of 17.483-279.720 μg·mL-1 and 4.088-65.406 μg·mL-1,respectively.The average recoveries were 104.27% with RSD 2.61%(n=9) for genistin and 97.16% with RSD 5.54%(n=9) for genistein.The contents of genistin and genistein in Moghania medicinal material from different habitants showed remarkable difference.Conclusion:The developed method is simple,well efficiency and precise,which can be used to determine content of genistin and genistein in Moghania medicinal material and provided the scientific basis for the quality evaluation as well as rational development and utilization of Moghania medicinal material.
keywords:Moghania medicinal material  genistin  genistein  HPLC  quality standard
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