| 薄层扫描法同时测定三七细粉中皂苷含量 |
| 投稿时间：2018-01-11 点此下载全文
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| 引用本文：何福龙,郑艳萍,任娟,金俊杰,白发平,蔡宝昌.薄层扫描法同时测定三七细粉中皂苷含量[J].中国现代中药,2018,20(8):975-978 |
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| 基金项目:国家中医药管理局公益性行业专项（201507002-2） |
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| 中文摘要:目的：建立同时测定三七细粉中人参皂苷Rb1、三七皂苷R1和人参皂苷Rg1含量的方法。方法：采用双波长薄层扫描法，吸附剂为硅胶G，展开剂为三氯甲烷-甲醇-水（13∶7∶2）10 ℃以下放置12 h的下层液，显色剂为10%硫酸乙醇溶液，110 ℃加热显色，测定波长510 nm，参比波长700 nm。结果：三种皂苷分别在0.504～5.04、0.501～5.01、0.492～4.92 μg范围呈良好的线性关系；平均回收率分别为96.40%、96.97%、98.97%,RSD分别为1.40% 、0.98%、1.70%（n=6）。结论：该方法操作简便、快速、高效、准确。 |
| 中文关键词:三七细粉 人参皂苷Rb1 三七皂苷R1 人参皂苷Rg1 薄层扫描法 |
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| Content Determination of Ginseng Saponin by TLC-scanning in Panax Notoginseng Powder |
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| Abstract:Objective：To establish a method for determination of ginsenosides content in Panax notoginseng powder.Methods：The double wavelength thin layer scanning method was applied with silica gel G as the adsorbent，the low layer of chloroform-methanol-water （13：7：2） under 10 ℃ placed 12 h was used as developing solvent，10% ethanol solution of sulfuric acid was used as the chromogenic agent with 110 ℃ heating，the determining wavelength was set at 510 nm，reference wavelength was 700 nm.Results：The three saponins showed a good linear relationship in the range of 0.504-5.04，0.501-5.01，0.492-4.92 μg. The average recoveries were 96.40%，96.77%，98.97%; RSD were 1.40%，0.98%，1.70%（n=6）.Conclusion：The established method is simple，rapid and accurate. |
| keywords:Panax notoginseng powder ginseng saponin Rb1 notoginsenoside R1 ginseng saponin Rg1 TLC-scanning |
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