三七叶、三七花质量标准研究
投稿时间:2018-03-22     点此下载全文
引用本文:赵振霞,王敏,段吉平,刘永利,庄爱爱.三七叶、三七花质量标准研究[J].中国现代中药,2018,20(8):979-983
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作者中文名作者英文名单位中文名单位英文名E-Mail
赵振霞 ZHAO Zhen-xia 河北省药品检验研究院,石家庄050011 Hebei Institute for Drug Control,Shijiazhuang 050011,China  
王敏 WANG Min 河北省药品检验研究院,石家庄050011 Hebei Institute for Drug Control,Shijiazhuang 050011,China  
段吉平 Duan Ji-ping 河北省药品检验研究院,石家庄050011 Hebei Institute for Drug Control,Shijiazhuang 050011,China  
刘永利 LIU Yong-li 河北省药品检验研究院,石家庄050011 Hebei Institute for Drug Control,Shijiazhuang 050011,China 刘永利,主任药师,研究方向:中药质量控制方法研究;Tel:(0311)85212008-8041,E-mail:liuyongli2008@126.com 
庄爱爱 ZHUANG Ai-ai 河北科技大学 化学与制药工程学院,石家庄050016 School of chemical and Pharmaceutical Engineering,Hebei University of Science and Technology,Shijiazhuang 050016,China  
基金项目:国家“重大新药创制”科技重大专项:2014ZX09304307-002。
中文摘要:目的:建立三七叶和三七花的质量标准。方法:用TLC法定性鉴别;按《中华人民共和国药典》方法对水分、总灰分、酸不溶性灰分进行测定;采用高效液相色谱法测定了人参皂苷Rb1;人参皂苷Rb2;人参皂苷Rb3三种成分的含量,用Thermo C18(4.6×250 mm,5 μm)色谱柱,以乙腈和水为流动相梯度洗脱,流速为1.0 mL·min-1,检测波长为203 nm,柱温30 ℃。结果:薄层鉴别方法斑点清晰,分离度好;三七叶和三七花水分的平均值为9.1%、10.0%,总灰分的平均值为10.1%、6.9%,酸不溶性灰分平均值为0.40%、0.062%;含量测定人参皂苷Rb1、人参皂苷Rb2和人参皂苷Rb3与相邻峰完全分离,线性关系良好,三七叶平均加样回收率(n=6)分别为101.5%、100.9%、99.5%;三七花平均加样回收率(n=6)分别为100.6%、98.6%、101.3%。结论:建立的方法可用于三七叶和三七花的质量控制。
中文关键词:薄层色谱法  高效液相色谱法  三七叶  三七花  人参皂苷Rb1  人参皂苷Rb2  人参皂苷Rb3
 
Quality Specification Study of Leaves and Flower Buds of Panax notoginseng
Abstract:Objective:To establish the quality standard for Leaves and flower buds of Panax notoginseng.Methods:The TLC was used for qualitative identification.According to the Chinese Pharmacopoeia methods,the moisture,total ash and acid insoluble ash were determined.The content analysis was performed on a Thermo C18 column(4.6×250 mm,5 μm),with acetonitrile(A)- water(B) as the mobile phase with gradient elution and the flow rate was 1 ml·min-1.The column temperatures was set at 30℃ and the detection wavelength was 203nm.Results:The spots on the TLC plate were clear and the resolution was well.For leaves and flower buds of P.notoginseng,the mean moisture were 9.1%,10.0%,the mean total ash were 10.1%,6.9%,the mean acid insoluble ash were 0.40%,0.062%.Ginsenoside Rb1,ginsenoside Rb2 and ginsenoside Rb3 were separated with chromatographic peak of consecutive.The linear relationships of calibration curves were favorable.The recoveries(n=6)of ginsenoside Rb1,ginsenoside Rb2 and ginsenoside Rb3 were 101.5%、100.9%、99.5% (leaves of P.notoginseng) and 100.6%、98.6%、101.3% (flower buds of P.notoginseng).The contents were probably identical in different batch of leaves of P.notoginseng.Conclusion:The established qualitative and quantitative analysis method is applicable for quality control of leaves and flower buds of P.notoginseng.
keywords:TLC  HPLC  leaves of Panax notoginseng  flower buds of Panax notoginseng  ginsenoside Rb1  ginsenoside Rb2  ginsenoside Rb3
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