文冠果壳提取物体外抑制HepG2细胞增殖活性部位筛选研究 |
投稿时间:2017-11-15 点此下载全文
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引用本文:张严磊,施欢贤,李世映,段金廒,宋忠兴,唐志书.文冠果壳提取物体外抑制HepG2细胞增殖活性部位筛选研究[J].中国现代中药,2018,20(9):1083-1086 |
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基金项目:陕西省重点产业创新链(群)——社会发展领域项目(2018ZDCXL-SF-01-06) |
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中文摘要:目的:筛选文冠果壳体外抑制肝癌HepG2细胞增殖活性部位。方法:分别用水、10%、30%、50%、70%、95%的乙醇水溶液,从文冠果壳中提取得到A、B、C、D、E和F 6个提取部位(XSHE),采用MTT法来筛选抑制肝癌细胞HepG2增殖的活性部位。结果:显示6个提取部位均有抑制HepG2细胞增殖作用,其中提取部位F(95%乙醇水溶液提取物)抑制HepG2细胞增殖作用最强,当95% XSHE给药质量浓度为75.00 μg·mL-1时,其对HepG2细胞增殖的抑制率高达(80.44±2.33)%,高于等浓度的阳性对照丝裂霉素C(MMc),且HepG2细胞在95% XSHE干预下形态发生变化,具体表现为细胞皱缩,细胞间隙变大,从培养板脱落等作用。结论:文冠果壳提取物在体外具有良好的抑制HepG2增殖的生物活性,为我们进一步探索文冠果壳提取物抗肿瘤作用机制奠定了基础。 |
中文关键词:文冠果壳提取物 抑制HepG2细胞增殖 筛选 |
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Screen of Activity Part of Inhibiting Proliferation of HepG2 Cell Lines from Xanthoceras sorbifolia Shell |
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Abstract:Objective:To screen the activity part of inhabiting the multiplication of HepG2 cell line from Xanthoceras sorbifolia shell.Method:X.sorbifolia shell was extracted using water,10%,30%,50%,70% and 95% ethanol,and six different parts A,B,C,D,E and F were obtained,which were exerted in the experiment of inhabiting the multiplication of HepG2 cell line by MTT method.Results:The results showed that all extracts of X.sorbifolia shell showed activity of inhibiting the proliferation of cells of HepG2,among them the section F possessed the strongest antitumor activity,the inhibition of part F reached as high as 80.44%.Conclusion:X.sorbifolia shell extract has good biological activity in inhibiting the proliferation of HepG2 in vitro,which has laid a foundation for further explore the anti-tumor mechanism of X.sorbifolia shell extract. |
keywords:extract of Xanthoceras sorbifolia shell inhibiting the proliferation of HepG2 cells screen |
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