不同产地抱茎苦荬菜的HPLC指纹图谱研究 |
投稿时间:2018-02-02 点此下载全文
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引用本文:杨非凡,张亚妮,武媛媛,李亚梅,王燕秋,李遇伯.不同产地抱茎苦荬菜的HPLC指纹图谱研究[J].中国现代中药,2018,20(9):1097-1104 |
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基金项目:吉林省科技发展计划项目(20170203006YY) |
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中文摘要:目的:建立抱茎苦荬菜药材的HPLC指纹图谱,为抱茎苦荬菜药材的质量控制和评价提供依据。方法:收集19批不同产地的抱茎苦荬菜,建立HPLC指纹图谱检测方法,使用中药色谱指纹图谱相似度评价系统计算相似度。结果:所建立的分析方法经过精密度、稳定性和重复性考察,均符合要求;指认了HPLC指纹图谱中的9个共有峰,经计算19批抱茎苦荬菜药材相似度高于0.95。结论:所建立的HPLC指纹图谱检测方法可为抱茎苦荬菜药材质量评价提供参考。 |
中文关键词:抱茎苦荬菜 指纹图谱 共有峰 相似度 仪器适用性 |
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HPLC Fingerprinting Analysis of Ixeris sonchifolia from Different Producing Areas |
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Abstract:Objective:The study is aimed to establish HPLC fingerprints of herbs of Ixeris sonchifolia,and provide a basis for quality control and evaluation of I.sonchifolia.Methods:19 batches of I.sonchifolia were collected from different regions and an HPLC fingerprinting method was established.The similarity of the HPLC fingerprints was calculated by using Similarity Evaluation System of Chromatographic Fingerprint of Traditional Chinese Medicine.Results:The analytical methods established in this study were based on precision,stability and repeatability studies,all met the requirements.Nine common peaks in HPLC fingerprints were identified,and the similarities of 19 batches of I.sonchifolia were higher than 0.95.Conclusion:The HPLC fingerprinting method established in this study can provide a reference for the quality evaluation of I.sonchifolia. |
keywords:Ixeris sonchifolia fingerprinting common peak similarity instrument suitability |
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