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作者中文名	作者英文名	单位中文名	单位英文名	E-Mail
丁万隆	DING Wan-long	中国医学科学院北京协和医学院药用植物研究所，北京100193	Institute of Medicinal Plant Development，Chinese Academy of Medical Sciences & Peking Union Medical College，Beijing 100193，China
王蓉	WANG Rong	中国医学科学院北京协和医学院药用植物研究所，北京100193	Institute of Medicinal Plant Development，Chinese Academy of Medical Sciences & Peking Union Medical College，Beijing 100193，China
郝单丽	HAO Dan-li	中国中医科学院中药研究所，北京100700	Institute of Chinese Materia Medica，China Academy of Chinese Medical Science，Beijing 100700，China
张晓云	ZHANG Xiao-yun	中国医学科学院北京协和医学院药用植物研究所，北京100193	Institute of Medicinal Plant Development，Chinese Academy of Medical Sciences & Peking Union Medical College，Beijing 100193，China
李勇	LI Yong	中国医学科学院北京协和医学院药用植物研究所，北京100193	Institute of Medicinal Plant Development，Chinese Academy of Medical Sciences & Peking Union Medical College，Beijing 100193，China	李勇，研究员，研究方向：药用植物病害生物防治；E-mail：liyong@ implad.ac.cn

基金项目:中国医学科学院医学与健康科技创新工程项目（2016-I2M-3-017）；国家中药标准化项目（ZYBZH-C-JL-27）

中文摘要:目的：明确12种市售微生物菌剂产品中分离菌株对人参黑斑病菌和人参灰霉菌的抑菌效果，为人参病害绿色防控提供参考依据。方法：采用稀释平板法从微生物菌剂中分离活体菌株；对峙培养法评价分离菌株的抑菌活性；十字交叉法测量菌落直径。结果：5种木霉菌剂产品中仅有1种分离到活体菌株，尽管该菌株对人参黑斑菌和人参灰霉菌的抑菌活性较高（抑菌率分别为65.18%和76.82%），但菌剂中活菌的分离数偏低。7种芽孢杆菌菌剂中均能分离到活体菌株，除微囊粒剂和水剂外，其他菌剂产品中活体菌株分离数均较高；上述分离菌株对人参黑斑菌和人参灰霉菌的抑菌活性低于木霉菌，抑菌率分别为27.21%～33.04%和10.74%～29.40%。结论：芽孢杆菌和木霉杀菌剂均具有防治人参病害的生防潜力，考虑菌剂产品的稳定性和环境耐受力，芽孢杆菌类微生物菌剂更适合人参病害的田间防控。

中文关键词:人参黑斑病人参灰霉菌芽孢杆菌木霉抑菌活性

Evaluation of Antagonistic Activity of Biocontrol Microbial Agents against Ginseng Diseases

Abstract:Objective：To ensure the inhibition of fungal and bacterial strains isolated from 12 commercial microbial agents against Alternaria panax and Botrytis cinera.Methods：The living strains were isolated from microbial agents by dilution plate method.Antagonistic activities were evaluated by plate confrontation culture method.The diameters of pathogens were measured by crossing method.Results：The living strain only isolated from 1 out of 5 Trichoderma agents.Though Trichoderma isolate shown high antagonistic activity against A.panax and B.cinera，with the inhibitive rate of 65.18% and 76.82%，respectively.However，the number of the living spores was very low.On the contrary，the living bacteria were successfully isolated from all 7 Bacillus agents，and except for microencapsulant and water agents，the number of the living bacteria isolated from the other Bacillus agents were significantly higher than that from Trichoderma agent.And the inhibitive rate of them against A.panax and B.cinera were varied from 27.21% to 33.04%，and from 10.74% to 29.40%，respectively.Conclusion：Bacillus agents and Trichoderma agents having biocontrol potential against A.panax and B.cinera.Considering the stability and environmental tolerance，Bacillus agents were more fitted for the biocontrol of ginseng diseases in the field.

keywords:Alternaria Panax Botrytis cinera Bacillus Trichoderma inhibitive activity

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