| 药用植物冬凌草高通量转录组测序与分析 |
| 投稿时间：2018-06-18 点此下载全文
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| 引用本文：陈延清,胡志刚,刘大会,黄必胜,刘迪.药用植物冬凌草高通量转录组测序与分析[J].中国现代中药,2018,20(12):1476-1482 |
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| 基金项目:湖北省教育厅科学技术研究项目（B2016077） |
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| 中文摘要:目的：得到冬凌草高通量转录组数据，为挖掘冬凌草二萜类生物合成途径关键基因，研究冬凌草EST-SSR分子标记提供数据来源。方法：利用Illumina HiSeq 4000高通量测序技术，采用Trinity的方法从头组装、序列拼接和去冗余处理；基于 BLAST 完成 Unigene 编码蛋白NR功能注释、GO 分类、KEGG 代谢通路注释和分类、功能基因挖掘，SSR 分子标记挖掘。结果：获得冬凌草叶与茎两种不同组织共12 GB转录组数据，得到3 7961个Unigene 基因，平均长度1063 bp；得到冬凌草叶和茎转录组有60条unigenes参与萜类化合物骨架合成、6条unigene参与各种萜类化合物合成，有26条unigene参与二萜化合物合成途径，叶与茎两种组织中有差异表达的基因4565个，其中在茎中表达较高的为1668个，在叶中表达较高的有2697个，与二萜类化合物合成相关的差异表达基因有15个。结论：本研究为冬凌草二萜化合物生物合成途径中关键基因的挖掘和分子育种提供了数据信息，为深入研究冬凌草中冬凌草甲素等有效成分的生物合成途径及其调控机制提供基础。 |
| 中文关键词:冬凌草 二萜合成 转录组分析 RNA-seq 差异基因表达分析 |
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| High-throughput Transcriptome Sequencing and Analysis of Isodon rubescens（Hemsl.）H.Hara |
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| Abstract:Objective：To obtain high-throughput transcriptome data of Isodon rubescens（Hemsl.）H.Hara，and provide data sources for the study of the key genes of diterpenoid biosynthetic pathway and EST-SSR molecular markers in I. rubescens.Methods：Illumina HiSeq 4000 high-throughput sequencing technology was used to obtain transcriptome data from the leaves and stems of adult I.rubescens by Trinity method for de novo assembly，sequence splicing，and de-redundant processing.Unigene-encoded proteins were based on BLAST，NR functional annotation，GO classification，KEGG metabolic pathway annotation.Moreover，EST-SSR molecular was preliminarily developed.Results：A total of 12 GB transcriptome data were obtained from the annual leaf and stem tissues，yielding 37，961 Unigene genes with average length of 1063 bp.There were 60 unigenes involved in terpenoid skeleton synthesis，6 unigenes involved in various terpenoids synthesis，and 26 unigenes involved in diterpenoid compound pathways stem from the leaf and stem.There were 4565 differentially expressed genes in the tissues，of which 1668 were highly expressed in stems and 2697 were highly expressed in leaves.There were 15 differentially expressed genes related to the synthesis of diterpenoids.Conclusion：This study provides data for the key genes in diterpenoid biosynthetic pathways and molecular breeding，providing a basis for in-depth study of biosynthetic pathways and regulatory mechanisms of oridonin and other active ingredients in I.rubescens. |
| keywords:Isodon rubescens diterpene synthesis transcriptome analysis RNA-seq differential gene expression analysis |
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