| 双波长HPLC同时测定八角莲中4′-去甲基鬼臼毒素鬼臼毒素 槲皮素 山柰素的含量 |
| 投稿时间：2018-06-24 点此下载全文
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| 引用本文：钟水生,顾炳仁,周坚,张华锋,王亚琼,郝刚.双波长HPLC同时测定八角莲中4′-去甲基鬼臼毒素鬼臼毒素 槲皮素 山柰素的含量[J].中国现代中药,2018,20(12):1565-1568 |
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| 中文摘要:目的：建立同时测定八角莲中木脂素类成分4′-去甲基鬼臼毒素、鬼臼毒素和黄酮类成分槲皮素、山柰素含量的方法。方法：采用Dikma C18 plus（250 mm×4.6 mm ，5 μm）色谱柱；流动相为甲醇-0.1%磷酸水溶液（50∶50）；流速为1 mL·min-1；检测波长分别为290、360 nm。结果：4′-去甲基鬼臼毒素、鬼臼毒素、槲皮素、山柰素分别在进样量0.294 4~3.680、0.961 6~12.020、0.086 3~1.079 3、0.102 3~1.279 μg与峰面积呈现良好的线性关系；4个成分各个水平的方法回收率（n=9）均在97.1~102.1%。结论：该方法简便、准确，重复性良好，适用于八角莲中4′-去甲基鬼臼毒素、鬼臼毒素、槲皮素、山柰素4种成分的同时测定，能为八角莲药材和饮片的质量控制和临床应用提供依据。 |
| 中文关键词:八角莲 鬼臼毒素 4′-去甲基鬼臼毒素 槲皮素 山柰素 双波长HPLC |
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| Simultaneous Determination of 4′-Demethylpodophyotoxin，Podophyllotoxin |
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| Abstract:Objective：To establish a dual-wavelength HPLC method for simultaneous determination of 4′-demethylpodophyotoxin，odophyllotoxin，quercetin and kaempferide in Dysosmae Pleianthae Radix et Rhizoma.Methods：A Dikma C18 plus column（250 mm×4.6 mm，5 μm）was adopted，the mobile phase was a mixture of methanol and 0.1% phosphate acid solution（50：50）at a flow rate of 1 mL·min-1.Result：Good linearities were observed within the scope of 0.294 4-3.68 μg for 4′-demethylpodophyotoxin，0.961 6-12.02 μg for podophyllotoxin，0.086 34-1.079 3 μg for quercetin and 0.102 3-1.279 μg for kaempferide，respectively.The recoveries for the four components at each level were all in the range between 97.1-102.1%.Conclusion：The method was validated to be simple，accurate and robust，and applicable for the simultaneous measurement of the four components，thus provide basis for the quality control of Dysosmae Pleianthae Radix et Rhizoma. |
| keywords:Dysosmae Pleianthae Radix et Rhizoma podophyllotoxin 4′-demethylpodophyotoxin quercetin kaempferide dual-wavelength HPLC |
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