| 人参全草免疫调节作用研究 |
| 投稿时间：2018-05-30 点此下载全文
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| 引用本文：任国庆,邹晓峰,韩亚男,窦德强.人参全草免疫调节作用研究[J].中国现代中药,2019,21(1):54-61 |
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| 摘要点击次数: 1062 |
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| 基金项目:国家自然科学基金（81773858） |
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| 中文摘要:目的：以林下山参为例，探究人参全草对小鼠免疫功能的影响。方法：选用昆明种小鼠，进行刀豆蛋白A（Con A）诱导的小鼠脾淋巴细胞转化实验、二硝基氟苯（DNFB）诱导小鼠迟发型超敏反应（DTH）实验、抗体生成细胞检测、血清溶血素测定、小鼠碳廓清实验、小鼠腹腔巨噬细胞吞噬鸡红细胞实验、自然杀伤细胞（NK细胞）活性测定，探讨林下山参全草是否具有调节免疫力功能的作用。结果：林下山参全草对实验动物胸腺指数、脾脏指数无明显影响。各实验剂量组的林下山参全草对DNFB诱导的小鼠DTH的形成无明显促进作用，中、高剂量组的林下山参全草对Con A诱导的小鼠脾淋巴细胞增殖能力显著增强（P＜0.01），低、中剂量组的林下山参全草对小鼠抗体生成细胞数量、血清溶血素抗体积数均有显著提高作用（P＜0.01），低剂量组的林下山参全草能明显增强小鼠碳廓清能力（P＜0.01），各剂量组的林下山参全草对小鼠腹腔巨噬细胞吞噬鸡红细胞的能力、NK细胞活性均未表现出明显提高作用。结论：林下山参全草具有一定的调节小鼠免疫的作用。 |
| 中文关键词:人参全草 林下山参 免疫调节 |
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| Studies on the Immunoregulation of Lin-Xia-Shan-Shen in Mice |
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| Abstract:Objective：To investigate the immune enhancement effect of the whole plant of ginseng in mice，take Lin-Xia-Shan Shen as an example.Methods：The spleen lymphocyte transformation experiment induced by Con A，the delayed type hypersensitivity（DTH）reaction induced by DNFB，the dectection of antibody-producing，and the determination of serum hemolysin experiments were carried out with the Kunming mice.Furthermore，the carbon clearance test，the phagocytosis effect of macrophages with chicken red blood cell experiments，and the determination of natural killer cell activity were also carried out in order to explore the immunoregulation effect of Lin-Xia-Shan-Shen.Results：There was no significant effect on the thymus index and spleen index of the experimental animals treated with Lin-Xia-Shan-Shen.The promotion effect on DTH reaction induced by DNFB experimental dose of mountain cultivated ginseng whole plant had no significant effect on the f were also not observed.The mice treated with middle and high doses of Lin-Xia-Shan-Shen possessed potent proliferation effect on the spleen lymphocyte in mice induced by Con A（P<0.01）.low and medium doses of mountain cultivated ginseng whole plant significantly increased The number cells of antibody and the accumulation value of anti-body in serum was increased significantly in the groups treated with low and middle dose of Lin-Xia-Shan-Shen（P<0.01）.Furthermore，the clearance ability of carbon particle was enhanced significantly in the low dose group（P<0.01）.However，the phagocytosis ability of macrophages，and the activity of NK cells were not increased obviously in all groups.Conclusion：The whole plant of Lin-Xia-Shan-Shen has a certain role in adjust immunity in mice. |
| keywords:whole plant of ginseng Lin-Xia-Shan-Shen immunoregulation |
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