加工方法对金银花质量的影响
投稿时间:2018-08-15     点此下载全文
引用本文:尚庆文,于盱,梁呈元,亓希武,房海灵,巢建国,李维林.加工方法对金银花质量的影响[J].中国现代中药,2019,21(1):76-81
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作者中文名作者英文名单位中文名单位英文名E-Mail
尚庆文 SHANG Qing-wen 江苏省中医院,江苏南京210029 Jiangsu Province Hospital of TCM,Nanjing 210029,China  
于盱 YU Xu 江苏省中国科学院植物研究所,江苏南京210014 Institution of Botany Jiangsu Province and Chinese Academy of Sciences,Nanjing 210014,China  
梁呈元 LIANG Cheng-yuan 江苏省中国科学院植物研究所,江苏南京210014 Institution of Botany Jiangsu Province and Chinese Academy of Sciences,Nanjing 210014,China  
亓希武 QI Xi-wu 江苏省中国科学院植物研究所,江苏南京210014 Institution of Botany Jiangsu Province and Chinese Academy of Sciences,Nanjing 210014,China  
房海灵 FANG Hai-ling 江苏省中国科学院植物研究所,江苏南京210014 Institution of Botany Jiangsu Province and Chinese Academy of Sciences,Nanjing 210014,China  
巢建国 CHAO Jian-guo 南京中医药大学,江苏南京210023 Nanjing University of Chinese Medicine,Nanjing 210023,China 巢建国,研究员,研究方向:药用植物资源及质量评价;E-mail:jgchao@njutcm.edu.cn 
李维林 LI Wei-lin 江苏省中国科学院植物研究所,江苏南京210014 Institution of Botany Jiangsu Province and Chinese Academy of Sciences,Nanjing 210014,China  
基金项目:江苏省林业三新工程项目(LYSX[2014]11);江苏省农业三新工程项目(SXGC[2014]319)
中文摘要:目的:研究不同加工方法对金银花中绿原酸、木犀草苷和氨基酸含量的影响。 方法:分别采用高效液相色谱法和GB/T 8314—2002茶-游离氨基酸测定不同加工方法处理的金银花中绿原酸、木犀草苷和氨基酸含量,记录金银花的外观、水浸出物、酸不溶性灰分和总灰分。结果:微波杀青后低温干燥的方法具有良好的外观性状,经微波杀青低温干燥处理后,绿原酸和木犀草苷的含量较高,氨基酸含量降低,绿原酸、木犀草苷和氨基酸含量之和最高(P<0.05),微波杀青低温干燥的总灰分均较低(P<0.05)。结论:微波杀青低温干燥法是合适的金银花加工方法,值得在种植区推广。
中文关键词:金银花  加工  绿原酸  木樨草苷  氨基酸  高效液相色谱法
 
Effect of Different Processing Methods on the Medicinal Quality of Lonicerae Flos
Abstract:Objective:To study the effects of different processing methods on the content of chlorogenic acid,luteolin and amino acids in Lonicerae Flos.Methods:The high performance liquid chromatography and GB/T8314-2002 tea-free amino acid were used to determine the content of chlorogenic acid,luteolin and amino acid in Flos Lonicerae treated by different processing methods.The appearance,water extract,acid-insoluble and total ash of Lonicerae Flos were recorded.Results:The method of the low temperature drying after microwave sterilization had good appearance.After microwave drying and low temperature drying treatment,the contents of chlorogenic acid and luteolin increased,the amino acid content decreased,and the sum of chlorogenic acid,luteolin and amino acid content was the highest(P<0.05).The total ash of Lonicerae Flos treated by low temperature drying after microwave sterilization decreased(P<0.05).Conclusion:The low temperature drying after microwave sterilization method is a suitable method for processing Lonicerae Flos,which is worthy of promotion in Lonicerae Flos planting areas.
keywords:Lonicerae Flos  processing  chlorogenic acid  lignin  amino acid  HPLC
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