| Box-Behnken响应面法优选地芍苣冬颗粒提取工艺 |
| 投稿时间：2018-06-25 点此下载全文
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| 引用本文：李玉婷,张刚,姜林,郝梦,依孜提·帕力哈提,刘怡,张丽娟,张良,唐梅.Box-Behnken响应面法优选地芍苣冬颗粒提取工艺[J].中国现代中药,2019,21(1):90-94 |
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| 基金项目:新疆地产中药民族药新药研发项目（2017-06-02） |
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| 中文摘要:目的：采用Box-Behnken响应面法优选地芍苣冬颗粒的提取工艺。方法：通过单因素试验考查浸泡时间、提取时间、加水倍数和提取次数对地芍苣冬颗粒中天麻素、芍药苷含量的影响。基于单因素的结果，应用Box-Behnken响应面法考察浸泡时间、提取时间、加水倍数３个因素对地芍苣冬颗粒中天麻素、芍药苷含量以及离体血管舒张率的影响，对结果进行二项式方程拟合，用响应面法预测最佳水提工艺。结果：二项式拟合方程预测性良好，理论预测值与实际值偏差较小。在最佳提取工艺条件（浸泡时间为1.5 h，加10倍量水提取1 h，共提取２次）下，天麻素平均质量分数为0.59 mg·g-1，芍药苷平均质量分数为6.35 mg·g-1，平均离体血管舒张率为71.03%。结论：此工艺稳定、方便，准确快速地优化地芍苣冬颗粒的提取工艺。 |
| 中文关键词:地芍苣冬颗粒 天麻素 芍药苷 离体血管舒张率 Box-Behnken响应面法 综合评分 |
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| Optimization of Dishaojudong Granule Extraction Process by Box-Behnken Response Surface Method |
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| Abstract:Objective：This paper aims to optimize the extraction process of Dishaojudong granule by using the Box-Behnken response surface method.Methods：The effects of immersion time，extraction time，extraction multiples and extraction times on the content of gastrotin，paeoniflorin were studied by single factor test.Based on the results of single factor，the effects of the study on the time of immersion，the time of extraction and the extraction multiples were studied.The effect of the three factors on the content of gastrotin，paeoniflorin and blood vessel diastolic rate in vitro was studied，the results were binomial equation fitting，by using the response surface method to predict the best water extraction process.Results：The predictive value of the binomial fitting equation was relatively low.In the best extraction technology conditions（soaking time for 1.5 hours，ten times the amount of water，extraction of 1 hour every time，extraction of twice），the mean content of gastrotin was 0.59 mg·g-1，the average content of paeoniflorin was 6.35 mg·g-1，and the average blood vessel diastolic rate in vitro was 71.03%.Conclusion：The method is stable and convenient and it can optimize the extraction process of the Dishaojudong granules accurately and rapidly. |
| keywords:Dishaojudong Granula gastrotin paeoniflorin blood vessel diastolic rate in vitro Box-behnken response surface method comprehensive score |
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