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作者中文名	作者英文名	单位中文名	单位英文名	E-Mail
王书云	WANG Shu-yun	河南大学药学院，河南开封475004	School of Pharmacy，Henan University，Kaifeng 475004，China
梁宏刚	LIANG Hong-gang	哈尔滨医科大学药化教研室/省部共建生物医药工程重点实验室，黑龙江哈尔滨150081	Department of Medicinal Chemistry and Natural Medicine Chemistry（State-Province Key Laboratories of Biomedicine-Pharmaceutics of China，Harbin Medical University，Harbin 150081，China
黄健	HUANG Jian	哈尔滨医科大学药化教研室/省部共建生物医药工程重点实验室，黑龙江哈尔滨150081	Department of Medicinal Chemistry and Natural Medicine Chemistry（State-Province Key Laboratories of Biomedicine-Pharmaceutics of China，Harbin Medical University，Harbin 150081，China
王金辉	WANG Jin-hui	哈尔滨医科大学药化教研室/省部共建生物医药工程重点实验室，黑龙江哈尔滨150081	Department of Medicinal Chemistry and Natural Medicine Chemistry（State-Province Key Laboratories of Biomedicine-Pharmaceutics of China，Harbin Medical University，Harbin 150081，China	王金辉，教授，研究方向：中药及民族药药效及物质基础研究；Tel：（451）86685745，E-mail：wangjh_716@163.com
杨宝峰	YANG Bao-feng	沈阳药科大学中药学院，辽宁沈阳110016	School of Traditional Chinese Materia Medica，Shenyang Pharmaceutical University，Shenyang 110016，China

基金项目:国家重大新药创制（2018ZX09305005）；护肝片标准化建设（ZYBZH-C-HLJ-15）；博士科研启动基金（CJ3050A24051）

中文摘要:目的：建立同时测定五味子中五味子醇甲、戈米辛D、五味子醇乙、当归酰戈米辛H、五味子甲素、戈米辛N、五味子乙素含量的HPLC方法。方法：采用SunFireTM C18色谱柱（150 mm×4.6 mm，5 μm），乙腈-水梯度洗脱，流速：1 mL·min-1，检测波长254 nm，柱温：30 ℃。结果：7个化合物在各自范围内均呈良好的线性关系，平均加样回收率为98.23%～100.51%，RSD为0.68%～1.80%。发现不同产地五味子中化合物的含量差别不大，7个木脂素的总质量分数最高为16.27 mg·g-1，最低为9.52 mg·g-1。结论：本实验建立的HPLC含量测定方法准确、可靠、重复性好，可用于五味子的质量控制。

中文关键词:五味子联苯环辛烯型木脂素类化合物高效液相色谱法含量测定

HPLC Simultaneous Determination of 7 Major Dibenzocyclooctadiene-type Lignans in fruits of Schisandrae chinensis

Abstract:Objective：To establish a method for the simultaneous determination of 7 dibenzocyclooctadiene-type lignans in Fructus Schisandrae Chinensis.Methods：The analysis was performed on a SunFireTM C18 column（150 mm×4.6 mm，5 μm）at 30°C，with the mobile phase acetonitrile-water system in a gradient elution manner at a flow rate of 1.0 ml/min，and the detection wavelength was set at 254 nm.Results：The linear range of the seven dibenzocyclooctadiene-type lignans were good in their own ranges，and their average recovery were between 98.23% and 100.51% with the RSDs of 0.68%-1.80%.There was no significant difference of the contents of seven compounds in Fructus Schisandrae Chinensis from different batches.The total content of seven investigated lignans was among 9.52-16.27 mg·g-1.Conclusions：The method is accurate，convenient and reproducible for the determination of 7 dibenzocyclooctadiene-type lignans in Fructus Schisandrae Chinensis.

keywords:Fructus Schisandrae Chinensis dibenzocyclooctadiene-type lignans HPLC quality control

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