| 大卫颗粒的质量标准提高 |
| 投稿时间：2018-09-19 点此下载全文
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| 引用本文：李晓,王学方,张丽先,李飞飞,宁二娟,魏悦.大卫颗粒的质量标准提高[J].中国现代中药,2019,21(4):517-522 |
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| 基金项目:河南省科技攻关项目（152102110115）；河南省科学院科研开发项目（18KF13003） |
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| 中文摘要:目的：提高大卫颗粒的质量标准。方法：对制剂中连翘、金银花、柴胡及甘草进行薄层色谱（TCL）鉴别；采用高效液相色谱法（HPLC）同时测定制剂中绿原酸、连翘酯苷A、黄芩苷的含量。结果：连翘、金银花、柴胡及甘草的TLC图斑点清晰，分离度好，阴性对照无干扰。绿原酸、连翘酯苷A、黄芩苷检测质量浓度线性范围分别为5.228～52.28（r=0.999 9）、5.92～59.20（r=0.999 8）、6.816～68.16 μg·mL-1（r=0.999 9）；精密度、稳定性、重复性试验的RSD<2.0%；平均加样回收率分别为101.84%（RSD=0.44%，n=6）、99.07%（RSD=0.79%，n=6）、101.30%（RSD=0.62%，n=6）。结论：该研究所建标准定性定量方法简便、专属性强、结果准确可靠，可用于大卫颗粒的质量控制。 |
| 中文关键词:大卫颗粒 薄层色谱鉴别 含量测定 质量控制 |
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| Improvement of Quality Standard of Dawei Granules |
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| Abstract:Objective：To improve the quality standard of Dawei granules.Methods：TLC was applied for qualitative identification of Forsythia suspensa，Lonicerae japonicae，Bupleurum chinense，Glycyrrhiza uralensis.The contents of baicalin，chlorogenic acidand and forsythoside A were determined by HPLC.Results：TLC spots of F.suspensa，L.japonicae，B.chinense and G.uralensis were clear and well-separated without negative interference.The linear ranges of chlorogenic acidand，forsythoside A and baicalin were 5.228-52.28 μg·mL-1（r=0.999 9），5.92-59.20 μg·mL-1（r=0.999 8）and 6.816-68.16 μg·mL-1（r=0.999 9）.RSDs of precision，stability and reproducibility tests were all lower than 1.0 %.The average sample adding recovery rates of chlorogenic acidand，forsythoside A and baicalin were 101.84 %（RSD=0.44%，n=6），99.07 %（RSD=0.79%，n=6）and 101.30 %（RSD=0.62%，n=6），respectively.Conclusions：This method is accurate，sensitive，simple and specific in good repeatability，which is suitable for quality control of Dawei granules. |
| keywords:Dawei granules TLC identification Content determination Quality standard |
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