Efficacies Identification of Shenlian Extracts on Ox-LDL-induced Macrophage-derived Foam Cell Formation and Its Apoptosis and Comparison of Its Fractions
Abstract:Objective:To study the effects of Shenlian on ox-LDL-induced macrophage-derived foam cell formation and its apoptosis and comparison of its fractions.Methods:Peritoneal macrophages were collected from the C57BL/6J mice.After culturing for 72 hours,the peritoneal macrophages were randomly divided into 7 groups:negative control group(NC),model group(model,20 mg·L-1 ox-LDL),water-soluble extract of Salvia miltiorrhiza group(DS,4.32 mg·L-1+20 mg·L-1 ox-LDL),liposoluble extract of S.miltiorrhiza group(DZ,2.5 mg·L-1+20 mg·L-1 ox-LDL),liposoluble extract of Andrographis paniculata group(C,3.18 mg·L-1+20 mg·L-1 ox-LDL),and the Shenlian extract group(SL,10 mg·L-1+20 mg·L-1 ox-LDL),endoplasmic reticulum stress inducer group(TM,1 g·L-1).After treatment for 24 hours,the accumulation of lipids were stained of oil red oxygen.The intracellular calcium concentration was detected by flow cytometry and fluorescent probes.The cell apoptosis was detected by Annexin V-FITC double staining.The Caspase-3 activity was measured by chromatometry and the activation of casepase-12 was detected by Western blot.Results:Compared with the negative control group,both the modeling group and the endoplasmic reticulum stress inducer group significantly increased the accumulation of lipids,the rates of apoptosis,the activity of Caspase-3,the concentration of intracellular calcium and the activation of Caspase-12.Compared with the ox-LDL modeling group,the Shenlian extract and liposoluble extract of A.paniculata decreased the accumulation of lipids.The Shenlian extract and liposoluble extract of S.miltiorrhiza significantly inhibited peritoneal macrophages apoptosis and decreased the activity of Caspase-3,and effectively reducesed the concentration of intracellular calcium and the activation of Caspase-12.Conclusion:By targeting macrophages,SL extract may inhibit the apoptosis of peritoneal macrophage-derived foam cells by alleviating ox-LDL-induced endoplasmic reticulum stress.