不同产地赤芝药材的HPLC指纹图谱建立及聚类分析 |
投稿时间:2018-08-23 点此下载全文
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引用本文:段晓颖,陈霄,马秋莹,牛晓静,翟海容.不同产地赤芝药材的HPLC指纹图谱建立及聚类分析[J].中国现代中药,2019,21(5):609-615 |
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基金项目:河南省基础与前沿项目(152300410105) |
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中文摘要:目的:建立赤芝药材的高效液相指纹图谱并进行聚类分析。方法:收集不同产地、不同批号的10批赤芝药材,以灵芝酸A、灵芝酸G为参照物,采用ZORBAX SB-C18(250 mm×4.6 mm,5 μm)色谱柱;流动相:乙腈-0.2%磷酸水,梯度洗脱;流速1.0 mL·min-1;检测波长254 nm。利用“中药色谱指纹图谱相似度评价系统2004A”建立指纹图谱,评价图谱的相似性,并进行聚类分析。结果:建立了10批赤芝药材的HPLC指纹图谱,共标定31个共有色谱峰,方法学考察结果符合指纹图谱的技术要求,相同产地的药材基本可以聚为一类。结论:该方法稳定、可靠、重复性好,为灵芝药材的质量评价提供了新的方法。 |
中文关键词:赤芝药材 指纹图谱 聚类分析 |
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HPLC Fingerprint of Ganoderma lucidum from Differents Origins and Establishment of Clustering Analysis |
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Abstract:Objective:To establish an HPLC fingerprint of Ganoderma lucidum.Method:10 batches of G.lucidum from different origins and batches were collected,ganoderic acid A and ganoderic acid were used as reference materials,the HPLC method was used in the study with ZORBAX SB-C18(250 mm×4.6 mm,5 μm)column and mobile phase of acetonitrile-0.2% phosphoric acid water,the flow rate was 1.0 mL·min-1,and detection wavelength was 254 nm.The similarity of the obtained fingerprints were evaluated by the Chinese Medicine Chromatographic Fingerprint Similarity Evaluation System 2004A.Results:The 10 batch of G.lucidum HPLC fingerprints were established,a total of 31 common peaks were calibrated,the method was validated.The medicinal materials of the same origin could be basically grouped into one class.Conclusion:The method is stable,reliable,reproducible,and provides a new method to evaluate the quality of G.lucidum. |
keywords:Ganoderma lucidum fingerprinting cluster analysis |
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