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作者中文名	作者英文名	单位中文名	单位英文名	E-Mail
赵斌斌	ZHAO Bin-bin	广西卫生职业技术学院，广西南宁530023	Guangxi Medical College，Guangxi Nanning 530023，China
周小雅	ZHOU Xiao-ya	广西卫生职业技术学院，广西南宁530023	Guangxi Medical College，Guangxi Nanning 530023，China	周小雅，副教授，研究方向：制剂工艺、质量管理；Tel：（0771）5640155，E-mail：603832282@qq.com
赖飞娥	LAI Fei-e	广西卫生职业技术学院，广西南宁530023	Guangxi Medical College，Guangxi Nanning 530023，China
常雪松	CHANG Xue-song	广西卫生职业技术学院，广西南宁530023	Guangxi Medical College，Guangxi Nanning 530023，China
黄敏	HUANG Min	广西中医药大学附属瑞康医院，广西南宁530011	Afilliated Ruikang Hospital of Guangxi University of Chinese Medicine，Guangxi Nanning 530011，China
梁国成	LIANG Guo-cheng	广西中医药大学附属瑞康医院，广西南宁530011	Afilliated Ruikang Hospital of Guangxi University of Chinese Medicine，Guangxi Nanning 530011，China
陈壮	CHEN Zhuang	广西中医药大学附属瑞康医院，广西南宁530011	Afilliated Ruikang Hospital of Guangxi University of Chinese Medicine，Guangxi Nanning 530011，China

基金项目:2016年度广西高校中青年教师基础能力提升项目（KY2016YB736）

中文摘要:目的：用HPLC同时测定金匮薯蓣膏中甘草苷、甘草酸、薯蓣皂苷的含量，建立金匮薯蓣膏的质量控制方法，并以此为标准，用正交试验设计考察金匮薯蓣膏提取过程，为建立金匮薯蓣膏的生产工艺与质量控制提供依据。方法：以十八烷基硅烷键合硅胶色谱柱，乙腈-0.05％磷酸溶液为流动相，以1.0 mL·min-1的流速进行梯度洗脱，分别于237、202 nm波长处检测吸收峰；以L9（34）正交试验设计考察提取过程中水用量、煎煮时间、煎煮次数等因素对甘草苷、甘草酸、薯蓣皂苷含量的影响。结果：水提取过程以头煎水量6倍，头煎90 min，二煎水量6倍，二煎时间60 min为好。结论：水提取过程的工艺条件可为建立金匮薯蓣膏生产工艺规程提供依据，含量检测方法可靠、简便，可同时检测3种主要有效成分含量。

中文关键词:金匮薯蓣膏甘草苷甘草酸薯蓣皂苷

An Investigation on Extraction Technology of “JINKUI common Yam Ointment” by Multi-index Orthogonal Test Method

Abstract:Objective：To determine the contents of glycyrrhizin，glycyrrhizic acid and dioscin in “JINKUI common yam ointment” by HPLC，establish its quality control method，use this quality control method，investigate the extraction process of “JINKUI common yam ointment”by orthogonal experimental design.To provide basis for the production process and quality control of“JINKUI common yam ointment” Methods：The HPLC method was applied in the study with octadecylsilical gel column，the mobile phase was acetonitrile and 0.05% phosphoric acid with flowrate of 1.0 mL·min-1，the detection wavelengths were set at 202，and 237 nm，respectively.L9（34）orthogonal design was applied to investigate the effect of water amount，decocting time based on glycyrrhizin，glycyrrhizic acid and dioscin.Results：The best extraction process by water is 6 times water and 90 minutes in the first decoction，6 times water and 60 minutes in the second.Conclusion：The technological conditions of extraction process by water can provide the basis for establishing the technological process of“JINKUI common yam ointment”.The content determination method is reliable and simple，and can simultaneously detect the contents of three main active ingredients.

keywords:JINKUI common yam ointment glycyrrhizin glycyrrhizic acid dioscin

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