TLC Identification and Determination of Chlorogenic Acid and Habaroside in Active Part of Simiao Yongan Decoction
Abstract:Objective:To establish a TLC method for identification of active part and an HPLC method for determination of chlorogenic acid and habaroside in Si-Miao-Yong-An decoction by TLC.Methods:TLC was used to identify Radix Scrophulariae and Radix Glycyrrhizae,and HPLC was used to detect the content of chlorogenic acid and habaroside in decoction.Results:The fluorescence spots with decoction in the corresponding positions of Habaroside and ammonium glycyrrhizate were identified in TLC,and the spots were clear and the negative controls were not disturbed.Chlorogenic acid and habaroside had good linear relationship in the range of 0.195-1.560 μg·μL-1(r=0.999 9)and 0.019 2-0.384 0μg·μL-1(r=1.000 0)respectively,and the average recovery rate was 96.5%(n=9)and 97.8%(n=9),RSD was 0.9% and 1.1%,respectively.Conclusion:The TLC identification and determination of chlorogenic acid and habaroside are strong in specificity,accurate,stable and reproducible,and can effectively control the quality of the active part of Si Miao Yong An decoction.