| 基于蛋白质组学技术的羚羊角的研究与分析 |
| 投稿时间：2018-12-19 点此下载全文
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| 引用本文：王玉团,石峰,杭宝建,梁翠荣,咸瑞卿,巩丽萍.基于蛋白质组学技术的羚羊角的研究与分析[J].中国现代中药,2019,21(8):1045-1048 |
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| 中文摘要:目的：基于蛋白质组学技术对羚羊角进行研究，为合理使用羚羊角提供参考。方法：将酶解后的羚羊角角壳和骨塞样品，通过Nano-LC-Orbitrap液质联用仪进行多肽分离及质谱数据采集，结合UniPortKB收录的Capra Hircus蛋白质组数据库，采用PEAKS Studio软件进行数据处理。结果：发现两者主要功能蛋白质及多肽无论在数量上还是种类方面既有某些相似性也具有明显的差异性。结论：骨塞及角壳一起磨成羚羊角粉使用具有一定的合理性，但必须将角壳和骨塞的用量比例控制在合理范围内，以确保羚羊角临床用药的安全性和有效性。 |
| 中文关键词:羚羊角 蛋白质组 角壳 骨塞 多肽 |
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| Research and Analysis of Antelope Horn Based on Proteomics Technology |
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| Abstract:Objective：To study antelope horn based on proteomics technology，and provide a reference for the rational use of it.Methods：Angular shell and bone plug were enzymatically hydrolyzed，polypeptides were separated and detected by Nano-LC-Orbitrap liquid chromatography，The data were analyzed by PEAKS Studio with the Capra Hircus protein group database of UniPortKB.Results：There were some similarities and differences both in quantity and species of the main functional proteins and polypeptides.Conclusions：It was reasonable to make the bone plug and angular shell into the same powder together.However，the amount of the angular shell and bone plug must be controlled in a reasonable proportion to ensure the clinical safety and effectiveness of antelope horn. |
| keywords:antelope horn proteomics angular shell bone plug polypeptide |
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