| 62份非PK型紫苏种质的挥发油GC-MS分析和新化学型发现 |
| 投稿时间：2019-02-16 点此下载全文
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| 引用本文：郭佳琪,李卫萍,沈奇,杨森,商志伟,郭宝林,葛菲.62份非PK型紫苏种质的挥发油GC-MS分析和新化学型发现[J].中国现代中药,2019,21(8):1068-1075 |
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| 基金项目:贵州特色植物种质资源利用与创新人才基地（RCJD2018-14）；北京工商大学北京市植物资源研究开发重点实验室开放课题（PRRD-2018-YB7） |
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| 中文摘要:目的：寻找国产紫苏新的化学型。方法：采用气味排除法，从800份紫苏种质中选出非PK化学型的种质，取叶片用水蒸气蒸馏法提取紫苏叶挥发油，经GC-MS对其化学成分进行分离和鉴定。结果：初筛获得62份非PK型紫苏种质，鉴定出3种新的单一成分构成化学型或亚型，包括1份PA-Ⅱ型种质，以高含量的D-柠檬烯为特点；3份PT-Ⅱ型种质，具有高含量（-）-胡椒酮，而不含有胡椒烯酮；2份PS型种质，以倍半萜成分为主，有以石竹烯氧化物为主的PS-c型和以（Z，E）α-法尼烯为主的PS-f型；混合化学型中，PAPK型3份，另有6种新混合型，包括PAPT型1份、PAPP型1份、PKPP型1份、PAPS型2份和PPPS型1份。结论：本研究丰富了紫苏属挥发油的化学型类型，为紫苏的研究和利用提供了珍贵的材料。 |
| 中文关键词:紫苏 挥发油 化学型 GC-MS PA-Ⅱ PT-Ⅱ |
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| GC-MS Analysis of Volatile Oil from 62 Non-PK Perilla Germplasms and Discovery of New Chemotypes |
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| Abstract:Objective：To search for new chemotypes of Perilla frutescens in China.Methods：Non-PK type’s germplasms were selected from 800 perilla germplasms by odor exclusion method，and the volatile oils from perilla leaves were extracted by steam distillation，and their chemical constituents were isolated and identified by GC-MS.Results：62 germplasms of non-PK perilla were obtained and three of them were identified as new single-component chemotypes or subtypes，such as 1 PA-Ⅱ germplasm with high content of D-limonene；3 PT-Ⅱ germplasms with high content of （-）-piperitone，but no piperitenone；2 PS germplasms mainly contained sesquiterpenes，PS-c type dominated by caryophyllene oxide and PS-f type dominated by（Z，E）-α-farnesene.Among the mixed chemotypes，there were 3 PAPK types and 6 new mixed types，including 1 PAPT type，1 PAPP type，1 PKPP type，2 PAPS types and 1 PPPS type.Conclusion：This study enriches the chemotypes of volatile oils of perilla and provides valuable materials for the research and utilization of perilla. |
| keywords:Perilla frutescens volatile oil chemotype GC-MS PA-Ⅱ PT-Ⅱ |
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