| 星点设计-响应面法优选苦参总生物碱水提醇沉工艺 |
| 投稿时间：2018-11-07 点此下载全文
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| 引用本文：覃辉.星点设计-响应面法优选苦参总生物碱水提醇沉工艺[J].中国现代中药,2019,21(8):1084-1088 |
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| 中文摘要:目的：优化苦参水提醇沉提取工艺，获取苦参总生物碱最佳的工业化提取工艺条件及技术参数。方法：采用Box-Benhnken中心组合设计，以苦参总生物碱提取率和浸膏得率的总权重为评价指标，对液料比、提取时间和醇沉浓度3个影响因素进行考察，以Design-Expert 8.05软件对实验数据作统计处理，优选确定最佳提取条件。结果：优化得到最佳工艺条件为加苦参饮片9倍量的水，回流提取2次，3 h/次，醇沉浓度为70%，此工艺条件下苦参中总生物碱的提取率可达1.62%，干膏得率为22.51%。结论：优选得到的工艺稳定、可行，可作为苦参工业化提取提供参考。 |
| 中文关键词:苦参 提取工艺 星点设计-响应面法 |
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| Optimazation of water Extraction and Alcohol Precipitation Total Alkaloid in Sophora flavescens by Star Point Design-response Surface Method |
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| Abstract:Objective：To optimize the extraction process of Sophora flavescens water extraction and alcohol precipitation，and obtain the best industrial extraction conditions and technical parameters of total alkaloids in S.flavescens.Methods：Box-Benhnken center combination design was used to evaluate the total weight of the total alkaloid extraction rate and the extraction yield.The factors including the liquid-to-liquid ratio，extraction time and alcohol concentration were investigated.The Design-Expert 8.05 software was applied to process the experimental data，and obtain the optimal extraction conditions.Results：The optimal process conditions were 9 times of water，decoction for 2 times，3 hours/time，and the concentration of alcohol was 70%.At this condition，the extraction rate of total alkaloids in S.flavescens could arrived 1.62%，the dry paste yield was 22.51%.Conclusion：the preferred process is stable and feasible，and can be used for industrial extraction of S.flavescens. |
| keywords:Sophora flavescens extraction technology central composite design-response surface method |
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