| 《中国药典》收载动物药材DNA条形码鉴定研究策略与方法 |
| 投稿时间：2019-02-13 点此下载全文
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| 引用本文：姚辉,张辉,陈士林.《中国药典》收载动物药材DNA条形码鉴定研究策略与方法[J].中国现代中药,2019,21(9):1137-1146 |
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| 摘要点击次数: 724 |
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| 基金项目:公益性行业科研专项（201507002-4-1-2）；四川省重点研发项目（2018SZ0061） |
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| 中文摘要:动物药材应用历史悠久，是中药资源的重要组成部分。近年来动物药材混伪品、替代品不断出现，严重影响了动物药材临床应用效果和用药安全。DNA条形码技术作为一种新兴的生物鉴定方法，可对《中华人民共和国药典》收载动物药材进行快速、准确的鉴定。本文就以COI为主体、ITS2为补充的动物药材DNA条形码鉴定体系的建立、动物药材DNA条形码鉴定中的关键技术和动物药材DNA条形码鉴定数据库进行介绍，为《中华人民共和国药典》收载动物药材DNA条形码鉴定研究提供参考。 |
| 中文关键词:动物药材 鉴定 DNA条形码 COI序列 |
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| Strategies and Methods of DNA Barcoding Medicinal Animals in Chinese Pharmacopoeia |
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| Abstract:Animal drugs are an important part of traditional Chinese medicine and have been used for thousands of years.Recently，the counterfeits and substitutes of medicinal animals emerged because of insufficiency of wild resources，which resulted in security risks of their applications.DNA barcoding is a novel technique for accurate and rapid identification of medicinal animals recorded in Chinese Pharmacopoeia by use of a short standard DNA sequence.This article reviewed the establishment of DNA barcoding system for medicinal animals based on COI as a core barcode and ITS2 as a complementary locus，the key techniques in the operating procedures and the database for the identification，which will provide references for the research of DNA barcoding medicinal animals in Chinese Pharmacopoeia. |
| keywords:medicinal animals identification DNA barcoding COI sequence |
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