| 基于DNA条形码分子技术的海龙及其混伪品鉴定研究 |
| 投稿时间：2019-02-15 点此下载全文
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| 引用本文：张红印,金平,刘冬,高晓晨,张辉.基于DNA条形码分子技术的海龙及其混伪品鉴定研究[J].中国现代中药,2019,21(9):1181-1185 |
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| 基金项目:公益性行业科研专项（201507002） |
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| 中文摘要:目的：基于DNA条形码分子技术对动物药海龙及其混伪品进行鉴别，建立一种统一的海龙鉴定技术手段。方法：对收集到的动物药海龙及其混伪品进行DNA提取、PCR扩增和双向测序检测，运用Codon Code Aligner进行序列拼接，采用MEGA 7.0软件进行序列比对分析，比较种内、种间序列差异，构建动物药海龙及其混伪品的系统发育树。结果：所有海龙药材及其混伪品均可以使用中药材DNA条形码分子鉴定技术进行鉴别，共得到7个物种129条序列，各物种之间序列区分明显。结论：中药材DNA条形码鉴定技术能够准确有效地鉴别动物药海龙及其混伪品，可以作为海龙类中药的统一鉴定方法，为海龙的临床用药安全提供了新的分子技术手段。 |
| 中文关键词:海龙 中药材DNA条形码鉴定技术 混伪品 |
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| Identification of Syngnathus and Its Adulterants Using DNA Barcoding |
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| Abstract:Objective：To establish a standard method to identify the traditional Chinese medicine of Syngnathus basing on the DNA barcoding.Methods：The Syngnathus and its adulterants were performed on DNA extraction，PCR amplification and bi-directional DNA sequencing，sequence assembly and consensus sequence generation were performed using the CodonCode Aligner.Neighbor-joining tree construction was carried out by MEGA 7.0.The intra-specific variation and the inter-specific divergence were calculated.Results：All the species could be identified based on DNA sequences database of TCM of deer which contained 129 samples of 7 species.Conclusion：DNA barcoding of TCM can accurately and effectively identify Syngnathus and its adulterants.It can be used as a unified identification method of Syngnathus and provide a new molecular technology for the clinical medication safety of TCM. |
| keywords:Syngnathus DNA barcoding of TCM adulterants |
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