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麝香的微型DNA条形码鉴别方法研究

投稿时间：2019-01-04 点此下载全文

引用本文：赵玉洋,周骏辉,袁媛,黄璐琦,金艳,蒋超.麝香的微型DNA条形码鉴别方法研究[J].中国现代中药,2019,21(9):1186-1191

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作者中文名	作者英文名	单位中文名	单位英文名	E-Mail
赵玉洋	ZHAO Yu-yang	中国中医科学院中药资源中心道地药材国家重点实验室培育基地，北京100700	State Key Laboratory Breeding Base of Dao-di Herbs，National Resource Center for Chinese Materia Medica，China Academy of Chinese Medical Sciences，Beijing 100700，China
周骏辉	ZHOU Jun-hui	中国中医科学院中药资源中心道地药材国家重点实验室培育基地，北京100700	State Key Laboratory Breeding Base of Dao-di Herbs，National Resource Center for Chinese Materia Medica，China Academy of Chinese Medical Sciences，Beijing 100700，China
袁媛	YUAN Yuan	中国中医科学院中药资源中心道地药材国家重点实验室培育基地，北京100700	State Key Laboratory Breeding Base of Dao-di Herbs，National Resource Center for Chinese Materia Medica，China Academy of Chinese Medical Sciences，Beijing 100700，China	袁媛，研究员，研究方向：中药鉴定与分子生药学；Tel：（010）64087649，E-mail：y_yuan0732@163.com
黄璐琦	HUANG Lu-qi	中国中医科学院中药资源中心道地药材国家重点实验室培育基地，北京100700	State Key Laboratory Breeding Base of Dao-di Herbs，National Resource Center for Chinese Materia Medica，China Academy of Chinese Medical Sciences，Beijing 100700，China
金艳	JIN Yan	中国中医科学院中药资源中心道地药材国家重点实验室培育基地，北京100700	State Key Laboratory Breeding Base of Dao-di Herbs，National Resource Center for Chinese Materia Medica，China Academy of Chinese Medical Sciences，Beijing 100700，China
蒋超	JIANG Chao	中国中医科学院中药资源中心道地药材国家重点实验室培育基地，北京100700	State Key Laboratory Breeding Base of Dao-di Herbs，National Resource Center for Chinese Materia Medica，China Academy of Chinese Medical Sciences，Beijing 100700，China	蒋超，助理研究员，研究方向：中药分子鉴定；Tel：（010）64087649，E-mail：jiangchao0411@163.com

基金项目:公益性行业科研专项（201507002-4-4）；中央本级重大增减支项目（2060302）；中央级公益性科研院所基本科研业务费专项（ZZ10-008）

中文摘要:目的：建立一种麝香药材的DNA分子鉴别方法，并与全长DNA条形码鉴别方法进行比较。方法：通过比较麝香及其混伪品的COI序列，设计出一对约180 bp的麝香微型DNA条形码鉴别引物，对麝香及其混伪品进行测序鉴定。结果：COI全长扩增引物能在72.1%的样本中扩出条带，而微型条形码引物能扩出所有麝香样本，且微型条形码片段物种鉴别能力与全长片段一致。使用建立的微型DNA条形码鉴别市售麝香样品，检出2批混伪品，其基原物种为鸡Gallus gallus。结论：微型DNA条形码可用于鉴别麝香原动物及药材。

中文关键词:麝香分子鉴定 DNA条形码微型条形码

Mini-DNA Barcoding Molecular Identification of Traditional Chinese Medicinal Moschus

Abstract:Objective：To identify accurately Moschus powder or the sachet by establish a new DNA mini barcoding，and compare the new method to cytochrome C oxidase subunit I（COI）DNA barcoding.Methods：46 musk deer products and 15 secretion samples（include the adulterants）were analyzed using different extraction kits，and by amplifying full-length（-700 bp）as well a mini-length（-180 bp）barcode belonging to COI.Results：In total，full barcode were generated for 72.1% of the analyzed products，and mini-barcode were successfully obtained from all Moschus samples.Mini-barcodes could also identification of Moschus at species level as the full-length barcode.Furthermore，two products labeled as Moschus were detected as Gallus gallus by the established mini-barcode identification method.Conclusion：The results demonstrated that mini-barcode provide a valid contribution to barcoding-based identification across severe degraded products.

keywords:moschus molecular authentication DNA barcoding mini-barcode

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