| 不同产地蟾酥药材HPLC指纹图谱及模式识别研究 |
| 投稿时间：2019-03-31 点此下载全文
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| 引用本文：成小兰,庞中化,王志刚,胡春萍,吕章明,曹鹏.不同产地蟾酥药材HPLC指纹图谱及模式识别研究[J].中国现代中药,2019,21(9):1262-1266 |
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| 基金项目:公益性行业科研专项（201507002）；国家自然科学基金项目（81622048） |
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| 中文摘要:目的：建立不同产地蟾酥的HPLC指纹图谱，并进行聚类分析和主成分分析，为蟾酥及混伪品鉴定提供快速可靠的分析方法。方法：收集10批不同产地的蟾酥样品，采用Waters MS C18色谱柱（150 mm×4.6 mm，5 μm），纯水-0.1%三氟乙酸（TFA）乙腈溶液为流动相梯度洗脱，流速1.0 mL·min-1，柱温30 ℃，检测波长296 nm。结果：提取10个色谱峰为指纹图谱共有峰，并采用对照品指认了3个主要色谱峰，其中以对照品蟾毒灵为参照峰，采用指纹图谱相似度评价软件、SIMCA软件进行相似度分析和主成分分析。方法学考察实验表明，所建立的指纹图谱方法重复性良好，简便可靠。不同来源的蟾酥药材相似度大于0.92。结论：本研究所建立的蟾酥药材指纹图谱方法快速准确，灵敏度高，可为不同产地蟾酥质量控制和混伪品鉴别提供有效的手段。 |
| 中文关键词:蟾酥 高效液相色谱 指纹图谱 相似度分析 主成分分析 |
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| HPLC Fingerprint Analysis and Pattern Recognition of Dried Venenum Bufonis from Different Origin |
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| Abstract:Objective：To establish HPLC fingerprint profiles of dried Venenum Bufonis from different producing areas，and provide a fast and reliable method for Venenum Bufonis identification.Methods：A total of 10 dried Venenum Bufonis samples from different origins were collected.The samples were analyzed with Waters MS C18 column（150 mm×4.6 mm，5 μm），and gradient eluted by water-0.1 % trifluoroacetic acid（TFA）acetonitrile solution at 1.0 ml/min.The column temperature was set at 30 ℃，and detect wavelength was 296 nm.Result：Ten chromatographic peaks were extracted as the common peaks of fingerprints，and three main chromatographic peaks were identified by reference，of which bufalin was used as the reference peak.Softwares such as fingerprint similarity evaluation，SIMCA were applied for similarity analysis and principal component analysis.The methodology study results showed that the established HPLC fingerprint method was simple and reliable.The similarity of Venenum Bufonis samples from different sources was greater than 0.92.Conclusion：The method established in this experiment is accurate and sensitive，can be used for quality control and identification of dried Venenum Bufonis. |
| keywords:Venenum Bufonis HPLC fingerprint similarity analysis principal component analysis |
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