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作者中文名	作者英文名	单位中文名	单位英文名	E-Mail
樊丛照	FAN Cong-zhao	新疆维吾尔自治区中药民族药研究所/国家中医药管理局新疆中药民族药资源重点实验室，新疆乌鲁木齐830002	Xinjiang Institute of Chinese Materia Medica and Ethical Materia Medica，State Administration of traditional Chinese medicine Key Laboratory of Traditional Chinese Medicine and Ethnic Medicine Resources，Urumqi 830002，China
郎乾坤	LANG Qian-kun	阿克苏市人民医院，新疆阿克苏843000	People′s Hospital of Akesu city，Akesu 843000，China
朱军	ZHU Jun	新疆维吾尔自治区中药民族药研究所/国家中医药管理局新疆中药民族药资源重点实验室，新疆乌鲁木齐830002	Xinjiang Institute of Chinese Materia Medica and Ethical Materia Medica，State Administration of traditional Chinese medicine Key Laboratory of Traditional Chinese Medicine and Ethnic Medicine Resources，Urumqi 830002，China
王果平	WANG Guo-ping	新疆维吾尔自治区中药民族药研究所/国家中医药管理局新疆中药民族药资源重点实验室，新疆乌鲁木齐830002	Xinjiang Institute of Chinese Materia Medica and Ethical Materia Medica，State Administration of traditional Chinese medicine Key Laboratory of Traditional Chinese Medicine and Ethnic Medicine Resources，Urumqi 830002，China
宋海龙	SONG Hai-long	新疆维吾尔自治区中药民族药研究所/国家中医药管理局新疆中药民族药资源重点实验室，新疆乌鲁木齐830002	Xinjiang Institute of Chinese Materia Medica and Ethical Materia Medica，State Administration of traditional Chinese medicine Key Laboratory of Traditional Chinese Medicine and Ethnic Medicine Resources，Urumqi 830002，China
李晓瑾	LI Xiao-jin	新疆维吾尔自治区中药民族药研究所/国家中医药管理局新疆中药民族药资源重点实验室，新疆乌鲁木齐830002	Xinjiang Institute of Chinese Materia Medica and Ethical Materia Medica，State Administration of traditional Chinese medicine Key Laboratory of Traditional Chinese Medicine and Ethnic Medicine Resources，Urumqi 830002，China	李晓瑾，研究员，研究方向：中药资源，Tel：（0991）2665614，E-mail：xjlxj@126.com

基金项目:国家现代农业产业技术体系建设专项（CARS-21）；中央本级重大增减支项目（2060302）

中文摘要:目的：从表型性状和分子学角度研究双色金鸡菊种质资源的多样性。方法：对双色金鸡菊19个采集地120份供试样品的10个表型性状进行统计，并用14条简单重复序列区间（ISSR）引物进行聚合酶链式反应（PCR）扩增，电泳检测后统计条带；使用SPSS 21.0等软件分析表型数据，PopGene 32分析条带统计结果。结果：表型性状中株高的遗传多样性信息指数最高，其次是花冠大小和花色差异、茎杆粗细；聚类结果可将19个采集地样品聚为两大类，其中引种高海拔山区的双色金鸡菊有聚在一起的趋势。ISSR-PCR扩增结果显示双色金鸡菊在物种水平上有较高的遗传多样性，不同产地有明显的遗传分化；聚类结果将19个采集地的双色金鸡菊分为5大类，皮山县等高海拔地区样品在遗传物质上显示出较强的地域性。结论：昆仑雪菊种质资源存在地域性的遗传分化，表型性状和ISSR分子标记可对昆仑雪菊的种质资源进行评价。

中文关键词:昆仑雪菊双色金鸡菊表形性状 ISSR 遗传多样性

Diversity Study of Kunlun Snow Chrysanthemum Base on Phenotypic Traits and Molecular Marker

Abstract:Objective：Study the diversity of Coreopsis tinctoria by phenotypic traits and molecular perspective.Methods：Ten phenotypic traits of 120 samples which collected from 19 area were counted，and 14 ISSR primers were used to amplify the samples.The phenotypic traits and electrophoretic bands were counted artificially，SPSS 21.0 and PopGene 32 were used to analyse the statistical data.Results：The phenotypic traits diversity index that plant height was the highest traits，followed by corolla size，color difference and stem thickness.19 samples were cluster into two categories by SPSS 21.0，and almost the samples from high-altitude gathering together.ISSR-PCR amplification results showed that Coreopsis tinctoria had higher genetic diversity at the species level，and there were obvious genetic differentiation from different areas.The clustering results divided the sample of 19 collections into five categories，and samples at high altitudes such as Pishan city showed strong regionality in genetic level.Conclusion：The germplasm resources of Coreopsis tinctoria have differentiated regionally，phenotypic traits and ISSR molecular markers can be used to evaluate the germplasm resources.

keywords:Kunlun snow chrysanthemum Coreopsis tinctoria phenotypic traits ISSR genetic diversity

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