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作者中文名	作者英文名	单位中文名	单位英文名	E-Mail
李铂	LI Bo	陕西中医药大学陕西省中药资源产业化协同创新中心，陕西咸阳712083	Shaanxi University of Chinese Medicine，Shaanxi Collaborative Innovation Center of Chinese Medicinal Resources Industrialization，Xianyang 712083，China
唐志书	TANG Zhi-shu	陕西中医药大学陕西省中药资源产业化协同创新中心，陕西咸阳712083	Shaanxi University of Chinese Medicine，Shaanxi Collaborative Innovation Center of Chinese Medicinal Resources Industrialization，Xianyang 712083，China	唐志书，教授，研究方向：中药剂型理论与新技术应用、中药资源生产与循环利用；Tel：（029）38182201，E-mail：tzs6565@163.com
王楠	WANG Nan	陕西中医药大学陕西省中药资源产业化协同创新中心，陕西咸阳712083	Shaanxi University of Chinese Medicine，Shaanxi Collaborative Innovation Center of Chinese Medicinal Resources Industrialization，Xianyang 712083，China
孙晓春	SUN Xiao-chun	陕西中医药大学陕西省中药资源产业化协同创新中心，陕西咸阳712083	Shaanxi University of Chinese Medicine，Shaanxi Collaborative Innovation Center of Chinese Medicinal Resources Industrialization，Xianyang 712083，China
黄文静	HUANG Wen-jing	陕西中医药大学陕西省中药资源产业化协同创新中心，陕西咸阳712083	Shaanxi University of Chinese Medicine，Shaanxi Collaborative Innovation Center of Chinese Medicinal Resources Industrialization，Xianyang 712083，China
宋忠兴	SONG Zhong-xing	陕西中医药大学陕西省中药资源产业化协同创新中心，陕西咸阳712083	Shaanxi University of Chinese Medicine，Shaanxi Collaborative Innovation Center of Chinese Medicinal Resources Industrialization，Xianyang 712083，China
杨新杰	YANG Xin-jie	陕西中医药大学药学院，陕西咸阳712046	College of Pharmacy，Shaanxi University of Chinese Medicine，Xianyang 712046，China
程虎印	CHENG Hu-yin	陕西中医药大学药学院，陕西咸阳712046	College of Pharmacy，Shaanxi University of Chinese Medicine，Xianyang 712046，China
黄柯朝	HUANG Ke-chao	陕西中医药大学药学院，陕西咸阳712046	College of Pharmacy，Shaanxi University of Chinese Medicine，Xianyang 712046，China

基金项目:国家中药标准化项目（ZYBZH-C-QIN-45）；中央本级重大增减支项目（2060302）；陕西省科技厅重点研发计划一般项目（2018SF-286）；国家现代农业产业技术体系建设专项（CARS-21）；2017年中医药公共卫生服务补助专项（财社［2017］66号）；陕西省教育厅专项科学研究计划项目（18JK0214）；咸阳市重大科技专项计划项目（2017k01-26）

中文摘要:目的：探究发光二极管（LED）不同光质对陕产重楼生理特性和成分积累的影响，初步揭示陕产重楼生长和次生代谢物积累所需最佳光质配比，为合理指导重楼人工种植提供理论依据。方法：以基质栽培的陕产重楼为试验材料，分别设置红光、蓝光、红光/蓝光4∶1、红光/蓝光2∶1、白光5种光质，考察不同光质处理对重楼生物量、叶绿素含量、蛋白质含量、超氧化物歧化酶（SOD）活性、总多糖和总皂苷含量的影响，初步揭示陕产重楼响应不同LED光质的生理机制。结果：与白光相比，红光/蓝光2∶1（2R1B）能够显著提高重楼叶片中叶绿素a、b含量，促进叶片蛋白质积累。2R1B处理30 d，叶片SOD酶活性相比对照组降低55.40%；2R1B处理20 d的重楼根茎中总多糖含量最高，为对照组的2.165倍。蓝光处理30 d的重楼根茎中总皂苷含量最高，为对照组的1.595倍。结论：综合多项指标，红光/蓝光2∶1对陕产重楼叶片中叶绿素含量、蛋白质含量和根茎中总多糖的积累具有显著的促进作用，重楼总皂苷的积累则以蓝光处理的效果为好。实际生产中应根据获取目标成分的不同合理选择最适LED光质处理。

中文关键词:重楼光质生理特性成分

Effects of Different LED Light Quality on Physiological Characteristics and Component Accumulation of Paris polyphylla in Shaanxi Province

Abstract:Objective：To investigate the effects of different light quality of light-emitting diodes on the physiological characteristics and component accumulation of Paris polyphylla in Shaanxi，reveal the optimal light-quality ratio for the growth and secondary metabolites accumulation in P.polyphylla，and provide reasonable guidance for its artificial cultivation.Methods：The red，blue，red：blue 4∶1，red：blue 2∶1，and white light quality were used to treat P.polyphylla cultured in the substrate，and investigate the biomass，chlorophyll content，superoxide dismutase activity，total polysaccharide and total saponin contents of P.polyphylla，reveal the physiological mechanism of P.polyphylla response to different LED light quality preliminary.Results：The contents of chlorophyll a，b and proteins in the leaves of P.polyphylla treated by red：blue 2∶1（2R1B）were promoted significantly compared with the white light.The SOD activity was decreased by 55.40% compared with the control treated by 2R1B for 30 d，the total polysaccharide content in the rhizome was the highest treated by 2R1B for 20 d，up to 2.165 fold compared with the control.However，the total saponin content in the rhizome reached its maximum treated by blue light for 30 d，which was up to 1.595 fold compared with the control.Conclusion：The red：blue 2∶1 light quality promotes the chlorophyll content，protein and total polysaccharides accumulation in P.polyphylla significantly.The accumulation of total saponins is better with blue light treatment.In actual production，the optimal light quality should be reasonably selected according to the different target components.

keywords:Paris polyphylla light quality physiological characteristics components

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