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作者中文名	作者英文名	单位中文名	单位英文名	E-Mail
李林轩	LI Lin-xuan	广西药用植物园/广西药用资源保护与遗传改良重点实验室，广西南宁530023	Guangxi Botanical Garden of Medicinal Plants，Guangxi Key Laboratory of Medicinal Resources Conservation and Genetic Improvement，Nanning 530023，China
梁莹	LIANG Ying	广西药用植物园/广西药用资源保护与遗传改良重点实验室，广西南宁530023	Guangxi Botanical Garden of Medicinal Plants，Guangxi Key Laboratory of Medicinal Resources Conservation and Genetic Improvement，Nanning 530023，China
秦双双	QING Shuang-shuang	广西药用植物园/广西药用资源保护与遗传改良重点实验室，广西南宁530023	Guangxi Botanical Garden of Medicinal Plants，Guangxi Key Laboratory of Medicinal Resources Conservation and Genetic Improvement，Nanning 530023，China
缪剑华	MIAO Jian-hua	广西药用植物园/广西药用资源保护与遗传改良重点实验室，广西南宁530023	Guangxi Botanical Garden of Medicinal Plants，Guangxi Key Laboratory of Medicinal Resources Conservation and Genetic Improvement，Nanning 530023，China
韦坤华	WEI Kun-hua	广西药用植物园/广西药用资源保护与遗传改良重点实验室，广西南宁530023	Guangxi Botanical Garden of Medicinal Plants，Guangxi Key Laboratory of Medicinal Resources Conservation and Genetic Improvement，Nanning 530023，China	韦坤华，研究员，研究方向：药用植物生物技术研究；E-mail：divinekh@163.com

基金项目:中央本级重大增减支项目（2060302）；广西科技计划项目（桂科AD17129044）；广西科技基地和人才专项（桂科2017AD19001）；广西医疗卫生适宜技术研究与开发项目（S201530）；“广西八桂学者”专项经费；桂林市科技计划项目重大专项（20180102-4）

中文摘要:目的：为推动广豆根种苗产业发展，获得规模化快速成苗生产技术。方法：以广豆根种子为外植体，按照种子-丛生芽-植株-炼苗移栽的繁育途径，对各阶段的培养基与其他影响因素开展研究，结果：结荚后生长90～120 d的种子在离体条件下均能萌发，其中结荚150～180 d的种子萌发效果最好，萌发率为95.7%；丛生芽增殖的最佳培养基为MS+1.5 mg·L-1 6-BA+0.3 mg·L-1 IAA +0.3 mg·L-1 KT，增值倍数约为7倍/30 d；20 d后转入4000 lux光照下培养10 d，获得叶片展开、木质化程度较高的健壮枝条；将枝条转接到生根培养基1/2MS+1.0 mg·L-1NAA+0.2 mg·L-1 IBA+0.5g·L-1 AC上，培养20 d后转入炼苗棚炼苗10 d，生根率达到95%。在南宁地区，生根苗以3—5月和10—11月为最佳移栽期，以通过泥炭土、碳化稻谷壳和珍珠岩的质量比例为4∶4.5∶1.5混合后为基质，移栽成活率可达95%。结论：通过组织培养技术建立了广豆根无菌播种规模化快速成苗生产技术，推动广豆根种苗产业发展。

中文关键词:广豆根种子丛生芽

Study on Industrialized Propagation Technology of Aseptic Seeding on Sophora tonkinensis Gagnep.

Abstract:Objective：To develop the seedling industry of Sophora tonkinensis Gapnep.Methods：The effects of culture medium and other factors on the growth and reproduction of seeds（seed，cluster bud，transplanting plant，precision seedling） were studied by using the seeds of Sophora tonkinensis Gapnep as explants.Results：After pod setting，seeds growth for 90-120 days could germinate in vitro，Among them，the Seed Germination Effect of 150-180 days is the best，and the germination rate is 95.7%；The optimum medium for multiplication of cluster buds was MS+1.5 mg·L-16-BA+0.3 mg·L-1IAA +0.3 mg·L-1KT，The multiplication coefficient was 7 for 30 d；After 20 days，the materials were cultured under 4000 lux light for 10 days，the clustered buds grew rapidly，proliferated more，and the buds were robust；And then，the branches were transfered to rooting medium of 1/2MS+NAA1.0 mg·L-1+0.2 mg·L-1 IBA+0.5g·L-1 AC for 20 d before transfered to seedling shed，the rooting rate reached 95% after 10 days of seedling refining.The best transplanting time of rooting is March to May and October to November in Nanning city.Peat soil，carbonized rice husk and perlite are mixed according to the mass ratio of 4∶4.5∶1.5，and the survival rate of rooting seedlings is 95%.Conclusion：through the tissue culture technology，the large-scale and rapid seedling production technology of aseptic sowing of guangdougen was established to promote the development of guangdougen seedling industry.

keywords:Sophora tonkinensis Gagnep. seeds cluster buds

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