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作者中文名	作者英文名	单位中文名	单位英文名	E-Mail
常艳旭	CHANG Yan-xu	天津中医药大学/天津市现代中药重点实验室，天津300193	Tianjin State Key Laboratory of Mordern Chinese Medicine，Tianjin University of TCM，Tianjin 300193，China
王涛	WANG Tao	天津中医药大学中药学院，天津300193	College of Chinese Materia Medica，Tianjin University of TCM，Tianjin 300193，China
李晋	LI Jin	天津中医药大学/天津市现代中药重点实验室，天津300193	Tianjin State Key Laboratory of Mordern Chinese Medicine，Tianjin University of TCM，Tianjin 300193，China
何俊	HE Jun	天津中医药大学/天津市现代中药重点实验室，天津300193	Tianjin State Key Laboratory of Mordern Chinese Medicine，Tianjin University of TCM，Tianjin 300193，China
金华	JIN Hua	天津中医药大学中药学院，天津300193	College of Chinese Materia Medica，Tianjin University of TCM，Tianjin 300193，China
马琳	MA Lin	天津中医药大学中药学院，天津300193	College of Chinese Materia Medica，Tianjin University of TCM，Tianjin 300193，China
杨雪晶	YANG Xue-jing	天津中医药大学/天津市现代中药重点实验室，天津300193	Tianjin State Key Laboratory of Mordern Chinese Medicine，Tianjin University of TCM，Tianjin 300193，China	杨雪晶，博士，研究方向：中药药效物质基础及质量评价；Tel：（0451）84838207，E-mail：yangxuejing@hrbcu.edu.cn

基金项目:中央本级重大增减支项目（2060302）；大学生创新创业训练计划项目（201510063026）

中文摘要:目的：建立同时测定桑椹饮片中新绿原酸、绿原酸、隐绿原酸、芦丁、异槲皮苷、紫云英苷和槲皮素7种成分含量的高效液相色谱（HPLC）测定方法，为评价中国不同地区桑椹饮片的质量提供技术手段。方法：采用ODS-C18（250 mm×4.6 mm，5 μm）色谱柱，水（含0.2%甲酸）-乙腈为流动相，梯度洗脱，流速为1 mL·min-1，检测波长为360 nm。结果：新绿原酸、绿原酸、隐绿原酸、芦丁、异槲皮苷、紫云英苷和槲皮素7种成分线性关系良好，加样回收率在95.0%~105%。不同地区使用的桑椹饮片中7种成分含量差异较大。结论：本研究建立了一种稳定、可靠的桑椹饮片中7种成分含量同时测定的高效液相色谱方法，为桑椹饮片质量研究提供参考。

中文关键词:桑椹高效液相色谱法含量测定

Simultaneous Determination of Seven Components in Mori Fructus Slice from Different Areas of China by HPLC

Abstract:Objective：To establish an HPLC-UV method for simultaneous determination of neochlorogenic acid，chlorogenic acid，cryptochlorogenic acid，rutin，quercetin，astragaline and quercetin for quality evaluation of Mori Fructus slice.Methods：The analysis was carried out on an ODS-2 column（250 mm×4.6 mm，5 μm）column with water（containing 0.2% formic acid）-acetonitrile as the mobile phase by RP-HPLC method.The detection wavelength was 360 nm and flow rate was 1 mL·min-1.Results：Seven components showed respectively a good linear relationship.The recoveries were 95.0%~105%.The contents of seven components in Mori Fructus slice from different areas was quite different.Conclusion：The simple and accurate HPLC-UV method was established and validated to determine the contents of seven components for quality evaluation of Mori Fructus.

keywords:Mori Fructus HPLC determination

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