以7种有效成分为指标评价内蒙古不同产地黄芪药材品质
投稿时间:2019-02-25     点此下载全文
引用本文:李紫岩,杨敏,王杰,张春红,李旻辉.以7种有效成分为指标评价内蒙古不同产地黄芪药材品质[J].中国现代中药,2020,22(1):65-73
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作者中文名作者英文名单位中文名单位英文名E-Mail
李紫岩 LI Zi-yan 包头医学院,内蒙古包头014060 Baotou Medical College,Baotou 014060,China  
杨敏 YANG Min 包头医学院,内蒙古包头014060 Baotou Medical College,Baotou 014060,China  
王杰 WANG Jie 包头医学院,内蒙古包头014060 Baotou Medical College,Baotou 014060,China  
张春红 ZHANG Chun-hong 包头医学院,内蒙古包头014060 Baotou Medical College,Baotou 014060,China 张春红,教授,研究方向:中蒙药药效学;Tel:(0472)7167890,E-mail:zchlhh@126.com 
李旻辉 LI Min-hui 包头医学院,内蒙古包头014060 Baotou Medical College,Baotou 014060,China 李旻辉,教授,研究方向:蒙中药资源保护与开发利用;Tel:(0471)6262232,E-mail:li_minhui@aliyun.com 
基金项目:内蒙古科技创新引导项目(KCBJ2018040);内蒙古自治区科技成果转化项目(CGZH2018174);内蒙古自治区科技计划项目(201701040);公益性行业科研专项(201507002);财政部中央本级专项(2060302);国家中药材产业技术体系综合试验站专项基金(CARS-21)
中文摘要:目的:选择7种有效成分为指标,对内蒙古地区不同产地的黄芪药材进行品质的初步评价。方法:采用高效液相色谱法对毛蕊异黄酮苷、芒柄花苷、毛蕊异黄酮、芒柄花素4种黄酮类成分进行含量测定,采用高效液相色谱联用蒸发光检测器法对黄芪甲苷、黄芪皂苷Ⅰ、黄芪皂苷Ⅱ 3种皂苷类成分进行含量测定,根据测得的40个产地黄芪样品中各指标成分含量,应用SPSS并以组间联结作为聚类方法,对不同产地黄芪样品进行聚类分析。结果:黄酮类、皂苷类成分含量测定方法均符合方法学考察要求,毛蕊异黄酮苷、芒柄花苷、毛蕊异黄酮、芒柄花素、黄芪皂苷Ⅰ、黄芪皂苷Ⅱ、黄芪甲苷的质量分数分别为0.021 6%~0.150 3%、0.00 26%~0.060 2%、0.000 2%~0.012 5%、0.000 3%~0.013 5%、0.023 9%~2.189 0%、0.041 3%~0.391 1%、0.040 7%~0.106 5%,聚类分析结果将不同产地黄芪样品分为3类,生长于阴山山脉山区的样品聚为一类,生长于阴山山脉山下周边农区的样品聚为一类,不在阴山山脉范围内的样品聚为一类。结论:本研究所采用的含量测定方法简单、稳定、准确,适用于黄芪中黄酮类、皂苷类成分的测定;生长在阴山山脉范围内的黄芪有效成分含量积累较多,品质较好,其中在包头市的固阳县与土默特右旗所产黄芪的品质最佳。
中文关键词:黄芪  黄酮  皂苷  含量测定  聚类分析
 
Evaluation of Quality of Astragali Radix in Different Producing Areas of Inner Mongolia with 7 Active Ingredients as Indicators
Abstract:Objective:Seven effective components of Astragali Radix were selected as the index components to determine the content and compare the quality of Astragali Radix in different regions of Inner Mongolia.Methods:HPLC-UV was used to determine the contents of 4 flavonoids (calycosin-7-glucoside,ononin,calycosin and formononetin).HPLC-ELSD was used to determine the contents of 3 saponins (astragaloside Ⅳ,astragaloside I and astragaloside Ⅱ).The cluster analysis of Astragali Radix samples from different regions was carried out by using SPSS and using the cluster connection as the cluster method.Results:The determination methods of flavonoids and saponins were investigated by means of methodology,and the results conformed to the requirements.Content range of calycosin-7-glucoside,ononin,calycosin,formononetin,astragaloside I,astragaloside Ⅱ and astragaloside Ⅳ are 0.021 6%-0.150 3%,0.002 6%-0.060 2%,0.000 2%-0.012 5%,0.000 3%-0.013 5%,0.023 9%-2.189 0%,0.041 3%-0.391 1% and 0.010 7%-0.106 5%,respectively.The cluster analysis results divided Astragali Radix samples from different habitats into three categories:the samples in the Yin Mountains range were classified into one category;the samples surrounding agricultural areas under the Yin Mountains were classified into one category;and the samples not within the range of the Yin Mountains range were classified into one category.Conclusion:The method is simple,stable and accurate.It is suitable for the determination of flavonoids and saponins in Astragali Radix.Astragali Radix growing in the range of Yin Mountains had a large accumulation of effective components and Astragali Radix produced in Guyang County and Tumed Right Banner had the best quality.
keywords:Astragali Radix  flavonoids  saponins  content determination  clustering analysis
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