| 以7种有效成分为指标评价内蒙古不同产地黄芪药材品质 |
| 投稿时间：2019-02-25 点此下载全文
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| 引用本文：李紫岩,杨敏,王杰,张春红,李旻辉.以7种有效成分为指标评价内蒙古不同产地黄芪药材品质[J].中国现代中药,2020,22(1):65-73 |
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| 基金项目:内蒙古科技创新引导项目（KCBJ2018040）；内蒙古自治区科技成果转化项目（CGZH2018174）；内蒙古自治区科技计划项目（201701040）；公益性行业科研专项（201507002）；财政部中央本级专项（2060302）；国家中药材产业技术体系综合试验站专项基金（CARS-21） |
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| 中文摘要:目的：选择7种有效成分为指标，对内蒙古地区不同产地的黄芪药材进行品质的初步评价。方法：采用高效液相色谱法对毛蕊异黄酮苷、芒柄花苷、毛蕊异黄酮、芒柄花素4种黄酮类成分进行含量测定，采用高效液相色谱联用蒸发光检测器法对黄芪甲苷、黄芪皂苷Ⅰ、黄芪皂苷Ⅱ 3种皂苷类成分进行含量测定，根据测得的40个产地黄芪样品中各指标成分含量，应用SPSS并以组间联结作为聚类方法，对不同产地黄芪样品进行聚类分析。结果：黄酮类、皂苷类成分含量测定方法均符合方法学考察要求，毛蕊异黄酮苷、芒柄花苷、毛蕊异黄酮、芒柄花素、黄芪皂苷Ⅰ、黄芪皂苷Ⅱ、黄芪甲苷的质量分数分别为0.021 6%～0.150 3%、0.00 26%～0.060 2%、0.000 2%～0.012 5%、0.000 3%～0.013 5%、0.023 9%～2.189 0%、0.041 3%～0.391 1%、0.040 7%～0.106 5%，聚类分析结果将不同产地黄芪样品分为3类，生长于阴山山脉山区的样品聚为一类，生长于阴山山脉山下周边农区的样品聚为一类，不在阴山山脉范围内的样品聚为一类。结论：本研究所采用的含量测定方法简单、稳定、准确，适用于黄芪中黄酮类、皂苷类成分的测定；生长在阴山山脉范围内的黄芪有效成分含量积累较多，品质较好，其中在包头市的固阳县与土默特右旗所产黄芪的品质最佳。 |
| 中文关键词:黄芪 黄酮 皂苷 含量测定 聚类分析 |
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| Evaluation of Quality of Astragali Radix in Different Producing Areas of Inner Mongolia with 7 Active Ingredients as Indicators |
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| Abstract:Objective：Seven effective components of Astragali Radix were selected as the index components to determine the content and compare the quality of Astragali Radix in different regions of Inner Mongolia.Methods：HPLC-UV was used to determine the contents of 4 flavonoids （calycosin-7-glucoside，ononin，calycosin and formononetin）.HPLC-ELSD was used to determine the contents of 3 saponins （astragaloside Ⅳ，astragaloside I and astragaloside Ⅱ）.The cluster analysis of Astragali Radix samples from different regions was carried out by using SPSS and using the cluster connection as the cluster method.Results：The determination methods of flavonoids and saponins were investigated by means of methodology，and the results conformed to the requirements.Content range of calycosin-7-glucoside，ononin，calycosin，formononetin，astragaloside I，astragaloside Ⅱ and astragaloside Ⅳ are 0.021 6%-0.150 3%，0.002 6%-0.060 2%，0.000 2%-0.012 5%，0.000 3%-0.013 5%，0.023 9%-2.189 0%，0.041 3%-0.391 1% and 0.010 7%-0.106 5%，respectively.The cluster analysis results divided Astragali Radix samples from different habitats into three categories：the samples in the Yin Mountains range were classified into one category；the samples surrounding agricultural areas under the Yin Mountains were classified into one category；and the samples not within the range of the Yin Mountains range were classified into one category.Conclusion：The method is simple，stable and accurate.It is suitable for the determination of flavonoids and saponins in Astragali Radix.Astragali Radix growing in the range of Yin Mountains had a large accumulation of effective components and Astragali Radix produced in Guyang County and Tumed Right Banner had the best quality. |
| keywords:Astragali Radix flavonoids saponins content determination clustering analysis |
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