| 大黄-积雪草不同比例配伍对大黄游离蒽醌溶出率的影响 |
| 投稿时间：2019-05-13 点此下载全文
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| 引用本文：韩怡,柳春娣.大黄-积雪草不同比例配伍对大黄游离蒽醌溶出率的影响[J].中国现代中药,2020,22(1):94-97 |
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| 基金项目:江苏省苏州市产业技术创新专项项目（SYSD2016161） |
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| 中文摘要:目的：研究大黄-积雪草不同比例配伍对大黄5个游离蒽醌（芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚）溶出率的影响。方法：采用高效液相色谱法分析大黄单提液及大黄-积雪草不同比例配伍混提液中5个游离蒽醌的含量。色谱条件：Thermo Hypersil BDS C18色谱柱（250 mm ×4.6 mm，5 μm）；流动相甲醇-0.1%磷酸溶液（85∶15），流速1 mL·min-1，柱温30 ℃，检测波长254 nm。结果：6种比例大黄-积雪草混提液游离蒽醌含量均小于大黄单提液，积雪草对大黄5个游离蒽醌的溶出影响明显。结论：综合考虑大黄单提液及各配伍比例游离蒽醌含量，认为大黄-积雪草临床最佳配伍比例为1∶5。 |
| 中文关键词:大黄-积雪草 配伍比例 游离蒽醌 高效液相色谱法 |
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| Effect of Different Proportion Compatibility of Rhei Rhizoma et Radix-Centellae Herba on Dissolution Rate of Rree Anthraquinone |
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| Abstract:Objective：To study the effects of different proportions of Rhei Rhizoma et Radix and Centellae Herba on the dissolution rate of 5 free anthraquinone （aloe-emodin，rhein，emodin，chrysophanol，physcion）.Methods：The content of 5 free anthraquinones in Rhei Rhizoma et Radix extract and Rhei Rhizoma et Radix and Centellae Herba were determined by HPLC.The tests were carried out by Thermo C18（250 mm×4.6 mm，5 μm）by isocratic elution with methanol and 0.1% phosphoric acid water solution as mobile phase at a flow rate of 1 mL·min-1，the column temperature was 30 ℃ and the detection wavelength was 254 nm.Results：The content of free anthraquinone in 6 kinds of Rhei Rhizoma et Radix and Centellae Herba mixture was lower than Rhei Rhizoma et Radix extract.The effect of Centellae Herba on the dissolution of 5 free anthraquinones from Rhubarb was obvious.Conclusion：The best compatibility ratio of Rhei Rhizoma et Radix and Centellae Herba is 1∶5 by comprehensive consideration of the content of free anthraquinone in Rhubarb single extract and each compatibility ratio. |
| keywords:Rhei Rhizoma et Radix and Centellae Herba compatibility ratio free anthraquinone HPLC |
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