中文摘要:目的:验证苏龙嘎-4颗粒涩肠、止泻、抗炎及镇痛功效。方法:小鼠按体质量随机分组,灌胃给予苏龙嘎-4颗粒,剂量(以生药计)分别为:0.16、0.33、0.65、1.30 g ·kg-1。采用炭末推进实验对苏龙嘎-4颗粒进行涩肠作用研究;以粪便观察法对苏龙嘎-4颗粒进行止泻作用研究;以二甲苯致小鼠耳肿胀实验对苏龙嘎-4颗粒进行抗炎作用研究;以醋酸扭体实验对苏龙嘎-4颗粒进行镇痛作用研究。结果:与空白组比较,苏龙嘎-4颗粒能缩短炭末在正常小鼠肠道内的推进距离(P<0.01),降低炭末推进率(P<0.05,P<0.01);与模型组比较,苏龙嘎-4颗粒能缩短炭末在蓖麻油所致腹泻小鼠肠道内的推进距离(P<0.001),降低炭末推进率(P<0.01,P<0.001);能减少蓖麻油致小鼠腹泻的稀便数(P<0.05,P<0.01);对硫酸镁致小鼠腹泻无显著抑制作用(P>0.05);能减少番泻叶致小鼠腹泻的稀便数(P<0.01);能减少扭体次数(P<0.05,P<0.01,P<0.001),提高扭体抑制率;能明显降低小鼠耳肿胀率(P<0.01,P<0.001)。结论:苏龙嘎-4颗粒具有明显的涩肠、止泻、抗炎及镇痛作用,值得进一步研发和推广。
Experimental Study on Anti-inflammatory,Analgesic and Antidiarrheal Effects of Sulongga-4 Granules
Abstract:Objective:To verify the effect of Sulongga-4 granules on the intestines,diarrhea,anti-inflammatory and analgesic effects.Methods:Mice were randomly divided into groups according to body weight. Sulongga-4 particles were intragastrically administered at doses raw medicine of 0.16,0.33,0.65 and 1.30 g·kg-1,respectively. The astringent effect of Sulongga-4 granules was studied by carbon powder propulsion test,the anti-diarrhea effect of Sulongga-4 granules was studied by fecal observation method,the analgesic effect of Sulongga-4 granules was studied by acetic acid writhing test,and the anti-inflammatory effect of Sulongga-4 granules was studied by xylene-induced ear swelling test.Results:Compared with the blank group,Sulongga-4 granules could shorten the advancement distance of carbon in the intestinal tract of normal mice (P<0.01),reduce charcoal propulsion rate(P<0.05,P<0.01). Compared with the model group,Sulongga-4 particles could shorten carbon. The propulsion distance in the intestine of mice with diarrhea caused by castor oil (P<0.01,P<0.001);the number of loose stools caused by castor oil in mice with diarrhea (P<0.05,P<0.01);the diarrhea caused by magnesium sulfate was not significant Inhibition (P>0.05);can reduce the number of loose stools caused by senna-induced diarrhea in mice (P<0.01);can reduce the number of writhing (P<0.05,P<0.01,P<0.001),improve the twist Body inhibition rate;can significantly reduce the swelling rate of mouse ears (P<0.01,P<0.001).Conclusion:Sulongga-4 granules have obvious effects on calf,diarrhea,anti-inflammatory and analgesic,which deserve further research and development.