不同栽培方式 产地蒙古黄芪中黄芪甲苷和总黄酮含量差异分析
投稿时间:2019-09-21     点此下载全文
引用本文:田文仓,冯紫薇,石福娟,石玥,张靖晗,丁一明,栾震,宋学武,赵婷,魏胜利.不同栽培方式 产地蒙古黄芪中黄芪甲苷和总黄酮含量差异分析[J].中国现代中药,2020,22(4):585-590
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作者中文名作者英文名单位中文名单位英文名E-Mail
田文仓 TIAN Wen-cang 北京中医药大学 中药学院,北京102488 College of Chinese Materia Medica,Beijing University of Chinese Medicine,Beijing 102488,China  
冯紫薇 FENG Zi-wei 北京中医药大学 中药学院,北京102488 College of Chinese Materia Medica,Beijing University of Chinese Medicine,Beijing 102488,China  
石福娟 SHI Fu-juan 北京中医药大学 中药学院,北京102488 College of Chinese Materia Medica,Beijing University of Chinese Medicine,Beijing 102488,China  
石玥 SHI Yue 北京中医药大学 中药学院,北京102488 College of Chinese Materia Medica,Beijing University of Chinese Medicine,Beijing 102488,China  
张靖晗 ZHANG Jing-han 北京中医药大学 中药学院,北京102488 College of Chinese Materia Medica,Beijing University of Chinese Medicine,Beijing 102488,China  
丁一明 DING Yi-ming 北京中医药大学 中药学院,北京102488 College of Chinese Materia Medica,Beijing University of Chinese Medicine,Beijing 102488,China  
栾震 LUAN Zhen 子洲县产业发展投资有限公司,陕西榆林718400 Zizhou County Industrial Development Investment Co.,Ltd.,Yulin 718400,China  
宋学武 SONG Xue-wu 中华人民共和国国家卫生健康委员会 药具管理中心,北京102488 Management Center for Drug Delivery,National Health Commission of People′s Republic of China,Beijing 102488,China  
赵婷 ZHAO Ting 北京中医药大学 中药学院,北京102488 College of Chinese Materia Medica,Beijing University of Chinese Medicine,Beijing 102488,China 赵婷,讲师,研究方向:中药质量评价与现代鉴定技术研究;Tel:(010)84738334,E-mail:zhaoting.china@gmail.com 
魏胜利 WEI Sheng-li 北京中医药大学 中药学院,北京102488 College of Chinese Materia Medica,Beijing University of Chinese Medicine,Beijing 102488,China 魏胜利,教授,研究方向:中药资源定向培育及中药资源综合开发;Tel:(010)84738334,E-mail:wsl7491@126.com 
基金项目:北京中医药大学自主课题(2019-JYB-XS-070)
中文摘要:目的:比较不同栽培方式、产地蒙古黄芪中黄芪甲苷、总黄酮含量差异。方法:采用HPLC、紫外分光光度法分别测定黄芪甲苷、总黄酮含量并对其结果进行方差分析。结果:育苗移栽和种子直播蒙古黄芪中黄芪甲苷平均质量分数分别为0.151%、0.245%,总黄酮平均质量分数分别为0.508%、0.541%。不同产地移栽蒙古黄芪中黄芪甲苷质量分数为0.061%~0.323%,总黄酮质量分数为0.376%~0.903%;不同产地种子直播蒙古黄芪中黄芪甲苷质量分数为0.161%~0.343%,总黄酮质量分数为0.399%~0.825%。结论:不同栽培方式的蒙古黄芪中黄芪甲苷含量差异有统计学意义(P<0.05),且种子直播黄芪大于育苗移栽黄芪,总黄酮含量差异无统计学意义;不同产地蒙古黄芪中黄芪甲苷和总黄酮含量差异有统计学意义。其中,育苗移栽蒙古黄芪中山西朔州市产区的黄芪甲苷含量最高,宁夏河川乡产区所含总黄酮含量最高。种子直播蒙古黄芪中,陕西子洲县西庄乡产区的黄芪甲苷含量最高,陕西府谷县产区的黄芪总黄酮含量最高。
中文关键词:蒙古黄芪  栽培方式  不同产地  黄芪甲苷  总黄酮
 
Difference Analysis of Astragaloside Ⅳ and Total Flavonoids Content from Astragalus membranaceus var. mongolicus under Different Cultivation Patterns and Producing Areas
Abstract:Objective:To compare the difference of astragaloside Ⅳ and total flavonoids content in Astragalus membranaceus var. mongolicus under different cultivation patterns and producing areas. Methods:The content of astragaloside Ⅳ and total flavonoids were determined by HPLC and UV spectrophotometry,and the results were analyzed by variance. Results:The average content of astragaloside Ⅳ in the seedling transplanted and direct-seeded A. membranaceus var. mongolicus were 0.151% and 0.245%,respectively. And the average content of total flavonoids were 0.508% and 0.541%,respectively. The content of astragaloside Ⅳ in seedling transplanted A. membranaceus var. mongolicus was between 0.061% and 0.323%,and the total flavonoids content was between 0.376% and 0.903%. The content of astragaloside Ⅳ in the direct-seeded A. membranaceus var. mongolicus of different producing areas was between 0.161% and 0.343%,and the total flavonoids content was between 0.399% and 0.825%. Conclusion:The content of astragaloside Ⅳ in A. membranaceus var. mongolicus under different cultivation patterns is significantly different(P<0.05),and the direct-seeded A. membranaceus var. mongolicus is larger than the seedling transplanted A. membranaceus var. mongolicus However,there is no significant difference in total flavonoids. The content of astragaloside Ⅳ and total flavonoids in A. membranaceus var. mongolicus. under different producing areas are significantly different. Among them,the content of astragaloside Ⅳ in seedling transplanted A. membranaceus var. mongolicus produced in Shuozhou of Shanxi was the highest,and the total flavonoids content of A. membranaceus var. mongolicus produced in Hechuan of Ningxia was the highest. The content of astragaloside Ⅳ in direct-seeded A. membranaceus var. mongolicus produced in Xizhuang Township,Zizhou,Shaanxi was the highest,and the total flavonoids content of A. membranaceus var. mongolicus produced in Fugu,Shaanxi was the highest.
keywords:Astragalus membranaceus (Fisch.) Bge. var. mongholicus (Bge.) Hsiao  cultivation patterns  different producing areas  astragaloside Ⅳ  total flavonoids
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